Carrier-specific unresponsiveness in reaginic antibody formation. II. Suppression of hapten and carrier responsiveness in presensitized rats

Fred Sanfilippo, David W. Scott

Research output: Contribution to journalArticlepeer-review

Abstract

By inducing carrier-specific tolerance to sheep γ-globulin (SGG) in rats challenged with TNP-SGG in alum, it has been possible to study the effect of helper T-cell Unresponsiveness on IgE anti-TNP antibody formation. Rats primed to either the carrier (SGG) or the hapten (TNP as TNP-KLH) were treated with a single high dose (10 mg) of soluble SGG resulting in a suppression of both IgE anti-TNP and anti-SGG antibody which was maintained following a normally immunogenic secondary challenge with TNP-SGG in alum. This suppression was relatively long lasting, with no detectable IgE responsiveness to hapten or carrier observed for up to 8 weeks after tolerance induction. Suppressed animals were able to respond to the hapten when challenged with TNP-KLH, indicating that the induced effect did not directly involve the IgE antibody producing cells, but rather the carrier-specific helper cells. These results parallel our previous findings for IgM and IgG responses in a similar system. Such relatively long lasting and easily induced suppression in IgE antibody formation to specific protein antigens in primed animals may eventually provide a clinically useful means of allergic desensitization to large protein allergens.

Original languageEnglish (US)
Pages (from-to)435-442
Number of pages8
JournalCellular Immunology
Volume28
Issue number2
DOIs
StatePublished - 1977
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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