Carrier proteins determine local pharmacokinetics and arterial distribution of paclitaxel

Mark A. Lovich, Chris Creel, Kristy Hong, Chao Wei Hwang, Elazer R. Edelman

Research output: Contribution to journalArticlepeer-review

Abstract

The growing use of local drug delivery to vascular tissues has increased interest in hydrophobic compounds. The binding of these drugs to serum proteins raises their levels in solution, but hinders their distribution through tissues. Inside the arterial interstitium, viscous and steric forces and binding interactions impede drug motion. As such, this might be the ideal scenario for increasing the amount of drug delivered to, and residence time within, arterial tissues. We quantified carrier-mediated transport for paclitaxel, a model hydrophobic agent with potential use in proliferative vascular diseases, by determining, in the presence or absence of carrier proteins, the maximum concentration of drug in aqueous solution, the diffusivity in free solution, and the diffusivity in arterial tissues. Whereas solubility of paclitaxel was raised 8.1-, 21-, and 57-fold by physiologic levels of α1-acid glycoproteins, bovine serum albumin, and calf serum over that in protein-free solution, diffusivity of paclitaxel in free solution was reduced by 41, 49, and 74%, respectively. When paclitaxel mixed in these solutions was applied to arteries both in vitro and in vivo, drug was more abundant at the tissue interface, but protein carriers tended to retain drug in the lumen. Once within the tissue, these proteins did not affect the rate at which drug traverses the tissue because this hydrophobic drug interacted with the abundant fixed proteins and binding sites. The protein binding properties of hydrophobic compounds allow for beneficial effects on transvascular transport, deposition, and distribution, and may enable prolonged effect and rationally guide local and systemic strategies for their administration.

Original languageEnglish (US)
Pages (from-to)1324-1335
Number of pages12
JournalJournal of Pharmaceutical Sciences
Volume90
Issue number9
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Carrier protein-mediated transport
  • Diffusion
  • Paclitaxel
  • Solubility
  • Vascular pharmacokinetics

ASJC Scopus subject areas

  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Carrier proteins determine local pharmacokinetics and arterial distribution of paclitaxel'. Together they form a unique fingerprint.

Cite this