Carrageenan as an adjuvant to enhance peptide-based vaccine potency

Yu Qian Zhang, Ya Chea Tsai, Archana Monie, Chien-Fu Hung, Tzyy Choou Wu

Research output: Contribution to journalArticle

Abstract

New innovative therapies are urgently required in order to combat the high mortality and morbidity associated with advanced cancers. Antigen-specific cancer immunotherapy using peptide-based vaccination has emerged as an attractive approach for the control of cancers due to its simplicity and easy preparation. However, such an approach requires the employment of suitable adjuvants. In the current study, we explored the employment of a sulfated polysaccharide compound from red algae, carrageenan (CGN) as an adjuvant for their ability to generate antigen-specific immune responses and antitumor effects in mice vaccinated with human papillomavirus type 16 (HPV-16) E7 peptide vaccine. We found that carrageenan can significantly enhance the E7-specific immune responses generated by E7 peptide vaccination via the TLR4 activation pathway. In addition, carrageenan could enhance the protective and therapeutic antitumor effects generated by E7 peptide vaccination against E7-expressing tumors. Furthermore, the observed enhancement was not restricted to E7 antigen but was also applicable to other antigenic systems. We also found that other structurally similar compounds to CGN, such as dextran, also generated similar immune enhancement. Thus, our data suggest that CGN and its structurally related compounds may serve as innovative adjuvants for enhancing peptide-based vaccine potency.

Original languageEnglish (US)
Pages (from-to)5212-5219
Number of pages8
JournalVaccine
Volume28
Issue number32
DOIs
StatePublished - Jul 2010

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Vaccine Potency
Subunit Vaccines
Carrageenan
carrageenan
adjuvants
peptides
vaccines
Vaccination
neoplasms
vaccination
antigens
Peptides
Neoplasms
immune response
Rhodophyta
Antigens
therapeutics
Investigational Therapies
Human papillomavirus 16
Papillomaviridae

Keywords

  • Carrageenan
  • E7
  • Human papillomavirus (HPV)
  • Peptide vaccination

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine
  • Medicine(all)

Cite this

Carrageenan as an adjuvant to enhance peptide-based vaccine potency. / Zhang, Yu Qian; Tsai, Ya Chea; Monie, Archana; Hung, Chien-Fu; Wu, Tzyy Choou.

In: Vaccine, Vol. 28, No. 32, 07.2010, p. 5212-5219.

Research output: Contribution to journalArticle

Zhang, Yu Qian ; Tsai, Ya Chea ; Monie, Archana ; Hung, Chien-Fu ; Wu, Tzyy Choou. / Carrageenan as an adjuvant to enhance peptide-based vaccine potency. In: Vaccine. 2010 ; Vol. 28, No. 32. pp. 5212-5219.
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