Cardiosphere-Derived Cells Demonstrate Metabolic Flexibility That Is Influenced by Adhesion Status

Junaid Afzal, Angel Chan, Mehmet Fatih Karakas, Kirubel Woldemichael, Styliani Vakrou, Yufan Guan, Jeffrey Rathmell, Richard Wahl, Martin Pomper, D. Brian Foster, Miguel A. Aon, Benjamin Tsui, Brian O'Rourke, M. Roselle Abraham

Research output: Contribution to journalArticle

Abstract

Adult stem cells demonstrate metabolic flexibility that is regulated by cell adhesion status. The authors demonstrate that adherent cells primarily utilize glycolysis, whereas suspended cells rely on oxidative phosphorylation for their ATP needs. Akt phosphorylation transduces adhesion-mediated regulation of energy metabolism, by regulating translocation of glucose transporters (GLUT1) to the cell membrane and thus, cellular glucose uptake and glycolysis. Cell dissociation, a pre-requisite for cell transplantation, leads to energetic stress, which is mediated by Akt dephosphorylation, downregulation of glucose uptake, and glycolysis. They designed hydrogels that promote rapid cell adhesion of encapsulated cells, Akt phosphorylation, restore glycolysis, and cellular ATP levels.

LanguageEnglish (US)
Pages543-560
Number of pages18
JournalJACC: Basic to Translational Science
Volume2
Issue number5
DOIs
StatePublished - Oct 1 2017

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Glycolysis
Cell Adhesion
Adenosine Triphosphate
Phosphorylation
Glucose
Adult Stem Cells
Hydrogels
Facilitative Glucose Transport Proteins
Oxidative Phosphorylation
Cell Transplantation
Energy Metabolism
Down-Regulation
Cell Membrane

Keywords

  • cardiosphere-derived cells (CDCs)
  • glycolysis
  • metabolism
  • oxidative phosphorylation (OxPhos)
  • sodium-iodide symporter (NIS)
  • SPECT imaging

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Afzal, J., Chan, A., Karakas, M. F., Woldemichael, K., Vakrou, S., Guan, Y., ... Abraham, M. R. (2017). Cardiosphere-Derived Cells Demonstrate Metabolic Flexibility That Is Influenced by Adhesion Status. JACC: Basic to Translational Science, 2(5), 543-560. DOI: 10.1016/j.jacbts.2017.03.016

Cardiosphere-Derived Cells Demonstrate Metabolic Flexibility That Is Influenced by Adhesion Status. / Afzal, Junaid; Chan, Angel; Karakas, Mehmet Fatih; Woldemichael, Kirubel; Vakrou, Styliani; Guan, Yufan; Rathmell, Jeffrey; Wahl, Richard; Pomper, Martin; Foster, D. Brian; Aon, Miguel A.; Tsui, Benjamin; O'Rourke, Brian; Abraham, M. Roselle.

In: JACC: Basic to Translational Science, Vol. 2, No. 5, 01.10.2017, p. 543-560.

Research output: Contribution to journalArticle

Afzal, J, Chan, A, Karakas, MF, Woldemichael, K, Vakrou, S, Guan, Y, Rathmell, J, Wahl, R, Pomper, M, Foster, DB, Aon, MA, Tsui, B, O'Rourke, B & Abraham, MR 2017, 'Cardiosphere-Derived Cells Demonstrate Metabolic Flexibility That Is Influenced by Adhesion Status' JACC: Basic to Translational Science, vol. 2, no. 5, pp. 543-560. DOI: 10.1016/j.jacbts.2017.03.016
Afzal J, Chan A, Karakas MF, Woldemichael K, Vakrou S, Guan Y et al. Cardiosphere-Derived Cells Demonstrate Metabolic Flexibility That Is Influenced by Adhesion Status. JACC: Basic to Translational Science. 2017 Oct 1;2(5):543-560. Available from, DOI: 10.1016/j.jacbts.2017.03.016
Afzal, Junaid ; Chan, Angel ; Karakas, Mehmet Fatih ; Woldemichael, Kirubel ; Vakrou, Styliani ; Guan, Yufan ; Rathmell, Jeffrey ; Wahl, Richard ; Pomper, Martin ; Foster, D. Brian ; Aon, Miguel A. ; Tsui, Benjamin ; O'Rourke, Brian ; Abraham, M. Roselle. / Cardiosphere-Derived Cells Demonstrate Metabolic Flexibility That Is Influenced by Adhesion Status. In: JACC: Basic to Translational Science. 2017 ; Vol. 2, No. 5. pp. 543-560
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