Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium

R. F. Levy, A. J. Serra, E. L. Antonio, L. Dos Santos, D. S. Bocalini, J. B. Pesquero, M. Bader, Vanessa Merino, H. A. De Oliveira, E. C. De Arruda Veiga, J. A. Silva, P. J.F. Tucci

Research output: Contribution to journalArticle

Abstract

Our aim was to evaluate whether endothelial overexpressing of the bradykinin B1 receptor could be associated with altered left ventricular and myocardial performance. Echocardiography and hemodynamic were employed to assess left ventricular morphology and function in Sprague Dawley transgenic rats overexpressing the endothelial bradykinin B1 receptor (Tie2B1 rats). The myocardial inotropism was evaluated on papillary muscles contracting in vitro. In Tie2B1 animals, an enlarged left ventricular cavity and lower fractional shortening coupled with a lower rate of pressure change values indicated depressed left ventricular performance. Papillary muscle mechanics revealed that both Tie2B1 and wild-type rat groups had the same contractile capacities under basal conditions; however, in transgenic animals, there was accentuated inotropism due to post-pause potentiation. Following treatment with the Arg9-BK agonist, Tie2B1 papillary muscles displayed a reduction in myocardial inotropism. Endothelial B1 receptor overexpression has expanded the LV cavity and worsened its function. There was an exacerbated response of papillary muscle in vitro to a prolonged resting pause, and the use of a B1 receptor agonist impairs myocardial inotropism.

Original languageEnglish (US)
Pages (from-to)925-932
Number of pages8
JournalPhysiological Research
Volume66
Issue number6
StatePublished - Jan 1 2017

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Bradykinin B1 Receptors
Transgenic Rats
Papillary Muscles
Muscle Contraction
Endothelium
Genetically Modified Animals
Mechanics
Left Ventricular Function
Sprague Dawley Rats
Echocardiography
Hemodynamics
Pressure

Keywords

  • Bradykinin receptor
  • Cardiac function
  • Cardiac structure
  • Myocardial function
  • Transgenic rat

ASJC Scopus subject areas

  • Physiology

Cite this

Levy, R. F., Serra, A. J., Antonio, E. L., Dos Santos, L., Bocalini, D. S., Pesquero, J. B., ... Tucci, P. J. F. (2017). Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium. Physiological Research, 66(6), 925-932.

Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium. / Levy, R. F.; Serra, A. J.; Antonio, E. L.; Dos Santos, L.; Bocalini, D. S.; Pesquero, J. B.; Bader, M.; Merino, Vanessa; De Oliveira, H. A.; De Arruda Veiga, E. C.; Silva, J. A.; Tucci, P. J.F.

In: Physiological Research, Vol. 66, No. 6, 01.01.2017, p. 925-932.

Research output: Contribution to journalArticle

Levy, RF, Serra, AJ, Antonio, EL, Dos Santos, L, Bocalini, DS, Pesquero, JB, Bader, M, Merino, V, De Oliveira, HA, De Arruda Veiga, EC, Silva, JA & Tucci, PJF 2017, 'Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium', Physiological Research, vol. 66, no. 6, pp. 925-932.
Levy RF, Serra AJ, Antonio EL, Dos Santos L, Bocalini DS, Pesquero JB et al. Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium. Physiological Research. 2017 Jan 1;66(6):925-932.
Levy, R. F. ; Serra, A. J. ; Antonio, E. L. ; Dos Santos, L. ; Bocalini, D. S. ; Pesquero, J. B. ; Bader, M. ; Merino, Vanessa ; De Oliveira, H. A. ; De Arruda Veiga, E. C. ; Silva, J. A. ; Tucci, P. J.F. / Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium. In: Physiological Research. 2017 ; Vol. 66, No. 6. pp. 925-932.
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