Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium

R. F. Levy; A. J. Serra; E. L. Antonio; L. Dos Santos; D. S. Bocalini; J. B. Pesquero; M. Bader; V. F. Merino; H. A. De Oliveira; E. C. De Arruda Veiga; J. A. Silva; P. J.F. Tucci

doi:10.33549/physiolres.933596

Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium

R. F. Levy, A. J. Serra, E. L. Antonio, L. Dos Santos, D. S. Bocalini, J. B. Pesquero, M. Bader, V. F. Merino, H. A. De Oliveira, E. C. De Arruda Veiga, J. A. Silva, P. J.F. Tucci

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Our aim was to evaluate whether endothelial overexpressing of the bradykinin B1 receptor could be associated with altered left ventricular and myocardial performance. Echocardiography and hemodynamic were employed to assess left ventricular morphology and function in Sprague Dawley transgenic rats overexpressing the endothelial bradykinin B1 receptor (Tie2B1 rats). The myocardial inotropism was evaluated on papillary muscles contracting in vitro. In Tie2B1 animals, an enlarged left ventricular cavity and lower fractional shortening coupled with a lower rate of pressure change values indicated depressed left ventricular performance. Papillary muscle mechanics revealed that both Tie2B1 and wild-type rat groups had the same contractile capacities under basal conditions; however, in transgenic animals, there was accentuated inotropism due to post-pause potentiation. Following treatment with the Arg⁹-BK agonist, Tie2B1 papillary muscles displayed a reduction in myocardial inotropism. Endothelial B1 receptor overexpression has expanded the LV cavity and worsened its function. There was an exacerbated response of papillary muscle in vitro to a prolonged resting pause, and the use of a B1 receptor agonist impairs myocardial inotropism.

Original language	English (US)
Pages (from-to)	925-932
Number of pages	8
Journal	Physiological Research
Volume	66
Issue number	6
DOIs	https://doi.org/10.33549/physiolres.933596
State	Published - 2017

Keywords

Bradykinin receptor
Cardiac function
Cardiac structure
Myocardial function
Transgenic rat

ASJC Scopus subject areas

Physiology

Access to Document

10.33549/physiolres.933596

Cite this

Levy, R. F., Serra, A. J., Antonio, E. L., Dos Santos, L., Bocalini, D. S., Pesquero, J. B., Bader, M., Merino, V. F., De Oliveira, H. A., De Arruda Veiga, E. C., Silva, J. A., & Tucci, P. J. F. (2017). Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium. Physiological Research, 66(6), 925-932. https://doi.org/10.33549/physiolres.933596

Levy, RF, Serra, AJ, Antonio, EL, Dos Santos, L, Bocalini, DS, Pesquero, JB, Bader, M, Merino, VF, De Oliveira, HA, De Arruda Veiga, EC, Silva, JA & Tucci, PJF 2017, 'Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium', Physiological Research, vol. 66, no. 6, pp. 925-932. https://doi.org/10.33549/physiolres.933596

@article{366ed008046844d5ba8d16f6c3933708,

title = "Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium",

abstract = "Our aim was to evaluate whether endothelial overexpressing of the bradykinin B1 receptor could be associated with altered left ventricular and myocardial performance. Echocardiography and hemodynamic were employed to assess left ventricular morphology and function in Sprague Dawley transgenic rats overexpressing the endothelial bradykinin B1 receptor (Tie2B1 rats). The myocardial inotropism was evaluated on papillary muscles contracting in vitro. In Tie2B1 animals, an enlarged left ventricular cavity and lower fractional shortening coupled with a lower rate of pressure change values indicated depressed left ventricular performance. Papillary muscle mechanics revealed that both Tie2B1 and wild-type rat groups had the same contractile capacities under basal conditions; however, in transgenic animals, there was accentuated inotropism due to post-pause potentiation. Following treatment with the Arg9-BK agonist, Tie2B1 papillary muscles displayed a reduction in myocardial inotropism. Endothelial B1 receptor overexpression has expanded the LV cavity and worsened its function. There was an exacerbated response of papillary muscle in vitro to a prolonged resting pause, and the use of a B1 receptor agonist impairs myocardial inotropism.",

keywords = "Bradykinin receptor, Cardiac function, Cardiac structure, Myocardial function, Transgenic rat",

author = "Levy, {R. F.} and Serra, {A. J.} and Antonio, {E. L.} and {Dos Santos}, L. and Bocalini, {D. S.} and Pesquero, {J. B.} and M. Bader and Merino, {V. F.} and {De Oliveira}, {H. A.} and {De Arruda Veiga}, {E. C.} and Silva, {J. A.} and Tucci, {P. J.F.}",

note = "Funding Information: We thank the Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de S{\~a}o Paulo (FAPESP) (Grant numbers 09/54225-8 and 2015/11028-9) and Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq) (Grant numbers 47840/2011-5 and 479395/2012-8) for funding this study. Publisher Copyright: {\textcopyright} 2017 Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic.",

year = "2017",

doi = "10.33549/physiolres.933596",

language = "English (US)",

volume = "66",

pages = "925--932",

journal = "Physiological Research",

issn = "0862-8408",

publisher = "Czech Academy of Sciences",

number = "6",

}

TY - JOUR

T1 - Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium

AU - Levy, R. F.

AU - Serra, A. J.

AU - Antonio, E. L.

AU - Dos Santos, L.

AU - Bocalini, D. S.

AU - Pesquero, J. B.

AU - Bader, M.

AU - Merino, V. F.

AU - De Oliveira, H. A.

AU - De Arruda Veiga, E. C.

AU - Silva, J. A.

AU - Tucci, P. J.F.

N1 - Funding Information: We thank the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Grant numbers 09/54225-8 and 2015/11028-9) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Grant numbers 47840/2011-5 and 479395/2012-8) for funding this study. Publisher Copyright: © 2017 Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic.

PY - 2017

Y1 - 2017

N2 - Our aim was to evaluate whether endothelial overexpressing of the bradykinin B1 receptor could be associated with altered left ventricular and myocardial performance. Echocardiography and hemodynamic were employed to assess left ventricular morphology and function in Sprague Dawley transgenic rats overexpressing the endothelial bradykinin B1 receptor (Tie2B1 rats). The myocardial inotropism was evaluated on papillary muscles contracting in vitro. In Tie2B1 animals, an enlarged left ventricular cavity and lower fractional shortening coupled with a lower rate of pressure change values indicated depressed left ventricular performance. Papillary muscle mechanics revealed that both Tie2B1 and wild-type rat groups had the same contractile capacities under basal conditions; however, in transgenic animals, there was accentuated inotropism due to post-pause potentiation. Following treatment with the Arg9-BK agonist, Tie2B1 papillary muscles displayed a reduction in myocardial inotropism. Endothelial B1 receptor overexpression has expanded the LV cavity and worsened its function. There was an exacerbated response of papillary muscle in vitro to a prolonged resting pause, and the use of a B1 receptor agonist impairs myocardial inotropism.

AB - Our aim was to evaluate whether endothelial overexpressing of the bradykinin B1 receptor could be associated with altered left ventricular and myocardial performance. Echocardiography and hemodynamic were employed to assess left ventricular morphology and function in Sprague Dawley transgenic rats overexpressing the endothelial bradykinin B1 receptor (Tie2B1 rats). The myocardial inotropism was evaluated on papillary muscles contracting in vitro. In Tie2B1 animals, an enlarged left ventricular cavity and lower fractional shortening coupled with a lower rate of pressure change values indicated depressed left ventricular performance. Papillary muscle mechanics revealed that both Tie2B1 and wild-type rat groups had the same contractile capacities under basal conditions; however, in transgenic animals, there was accentuated inotropism due to post-pause potentiation. Following treatment with the Arg9-BK agonist, Tie2B1 papillary muscles displayed a reduction in myocardial inotropism. Endothelial B1 receptor overexpression has expanded the LV cavity and worsened its function. There was an exacerbated response of papillary muscle in vitro to a prolonged resting pause, and the use of a B1 receptor agonist impairs myocardial inotropism.

KW - Bradykinin receptor

KW - Cardiac function

KW - Cardiac structure

KW - Myocardial function

KW - Transgenic rat

UR - http://www.scopus.com/inward/record.url?scp=85039166429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85039166429&partnerID=8YFLogxK

U2 - 10.33549/physiolres.933596

DO - 10.33549/physiolres.933596

M3 - Article

C2 - 28937259

AN - SCOPUS:85039166429

SN - 0862-8408

VL - 66

SP - 925

EP - 932

JO - Physiological Research

JF - Physiological Research

IS - 6

ER -

Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this