Carbon‐13 Nuclear Magnetic Resonance Spectroscopy Study of the Glucose Stress of Human Lenses

Ugur Camli, T. Murray, J. Aguayo, P. Tarantino, A. Hay, W. Stark, Lou‐Sing ‐S Kan

Research output: Contribution to journalArticle

Abstract

The sorbitol theory in diabetic cataractogenesis was based on sorbitol accumulation under glucose stress. Sorbitol accumulation was examined by 13C nuclear magnetic resonance spectroscopy (NMR) for the first time in matched human lenses incubated in 5.5 mM and 35.5 mM C‐1 13C‐enriched glucose up to 28 hours. The results showed that sorbitol and lactate in human tens can be detected at 35.5 mM, but not in 5.5 mM glucose solution. The glycolysis metabolic pathway of human lenses may be quite different from that of animals. The accumulation of metabolites can be traced and quantified by the intensities of 13C NMR peaks. Therefore, 13C NMR spectroscopy can be used as a valuable tool to investigate human lens carbohydrate metabolism non‐interventionally.

Original languageEnglish (US)
Pages (from-to)329-333
Number of pages5
JournalJournal of the Chinese Chemical Society
Volume36
Issue number4
DOIs
StatePublished - 1989

Keywords

  • C NMR
  • Diabetic cataractogenesis
  • Glucose stress
  • Human lenses
  • Lactace
  • Sorbitol

ASJC Scopus subject areas

  • Chemistry(all)

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