Carbon nanotubes induce secondary structure changes of bovine albumin in aqueous phase

Man Yang, Jie Meng, Xiaobo Mao, Yang Yang, Xuelian Cheng, Hui Yuan, Chen Wang, Haiyan Xu

Research output: Contribution to journalArticle

Abstract

Interaction of nanomaterials to protein molecules is one of the most important issues to deeply understand the influences of the nanomaterials upon physiological processes and protein functions. So far most of investigations focused on the protein molecules adsorbed on the nanomaterials surface, less is known about those in the aqueous phase (not absorbed). In this work, luminescent spectroscopy analysis, circular dichroism measurement, atomic force microscopy, matrix-assisted laser desorption/ionization time of flight mass spectrometry, isoelectric focusing and sulfate polyacrylamide gel electrophoresis were used to investigate the influence of oxidized water-soluble multiwalled carbon nanotubes (CNT) dispersing in aqueous solution upon the structures of bovine serum albumin (BSA) through co-incubation. We focused on BSA molecules that stayed in the aqueous phase instead of those adsorbed by CNT. Experimental results show that the fractions of β-sheet decreased from 33.3% to 29.8% and β-turn increased from 2% to 5% in reference with native BSA. There was a slight increase of α-helix and a slight reduction of random coil. BSA molecules that had been encountered with CNT and were left in the solution formed a loose and flatten morphology on graphite substrates instead of their native tight and round morphology observed by AFM. The value of isoelectric point for BSA after exposed to CNT moved towards to a higher pH position compared with native BSA. All together, it was concluded that the oxidized water-soluble multiwalled carbon nanotubes not only adsorb bovine serum albumin molecules to their surface, but also induces albumin molecules in the aqueous solution undergo secondary structure changes, which lead to a conformation change.

Original languageEnglish (US)
Pages (from-to)7550-7553
Number of pages4
JournalJournal of Nanoscience and Nanotechnology
Volume10
Issue number11
DOIs
StatePublished - Nov 2010
Externally publishedYes

Fingerprint

Carbon Nanotubes
Bovine Serum Albumin
albumins
Albumins
Carbon nanotubes
serums
carbon nanotubes
Molecules
Nanostructures
Nanostructured materials
Multiwalled carbon nanotubes (MWCN)
Proteins
molecules
proteins
Physiological Phenomena
Dichroism
atomic force microscopy
aqueous solutions
Polyacrylates
Electrophoresis

Keywords

  • Aqueous phase
  • Carbon nanotubes
  • Interaction
  • Protein
  • Secondary structure

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Chemistry(all)
  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Carbon nanotubes induce secondary structure changes of bovine albumin in aqueous phase. / Yang, Man; Meng, Jie; Mao, Xiaobo; Yang, Yang; Cheng, Xuelian; Yuan, Hui; Wang, Chen; Xu, Haiyan.

In: Journal of Nanoscience and Nanotechnology, Vol. 10, No. 11, 11.2010, p. 7550-7553.

Research output: Contribution to journalArticle

Yang, Man ; Meng, Jie ; Mao, Xiaobo ; Yang, Yang ; Cheng, Xuelian ; Yuan, Hui ; Wang, Chen ; Xu, Haiyan. / Carbon nanotubes induce secondary structure changes of bovine albumin in aqueous phase. In: Journal of Nanoscience and Nanotechnology. 2010 ; Vol. 10, No. 11. pp. 7550-7553.
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