Carbon nanotube-based substrates for modulation of human pluripotent stem cell fate

Marina V. Pryzhkova; Indrat Aria; Qingsu Cheng; Greg M. Harris; Xingjie Zan; Morteza Gharib; Ehsan Jabbarzadeh

doi:10.1016/j.biomaterials.2014.03.011

Carbon nanotube-based substrates for modulation of human pluripotent stem cell fate

Marina V. Pryzhkova, Indrat Aria, Qingsu Cheng, Greg M. Harris, Xingjie Zan, Morteza Gharib, Ehsan Jabbarzadeh

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

We investigated the biological response of human pluripotent stem cells (hPSCs) cultured on a carbon nanotube (CNT) array-based substrate with the long term goal to direct hPSC germ layer specification for a wide variety of tissue engineering applications. CNT arrays were fabricated using a chemical vapor deposition system allowing for control over surface roughness and mechanical stiffness. Our results demonstrated that hPSCs readily attach to hydrophilized and extracellular matrix coated CNT arrays. hPSCs cultured as colonies in conditions supporting self-renewal demonstrated the morphology and marker expression of undifferentiated hPSCs. Conditions inducing spontaneous differentiation lead to hPSC commitment to all three embryonic germ layers as assessed by immunostaining and RT-PCR analysis. Strikingly, the physical characteristics of CNT arrays favored mesodermal specification of hPSCs. This is contradictory to the behavior of hPSCs on traditional tissue culture plastic which promotes the development of ectoderm. Altogether, these results demonstrate the potential of CNT arrays to be used in the generation of new platforms that allow for precise control of hPSC differentiation by tuning the characteristics of their physical microenvironment.

Original language	English (US)
Pages (from-to)	5098-5109
Number of pages	12
Journal	Biomaterials
Volume	35
Issue number	19
DOIs	https://doi.org/10.1016/j.biomaterials.2014.03.011
State	Published - Jun 2014
Externally published	Yes

Keywords

Cell adhesion
Cytoskeleton
Differentiation
Human pluripotent stem cells
Multi-walled carbon nanotubes
Surface roughness

ASJC Scopus subject areas

Biophysics
Bioengineering
Ceramics and Composites
Biomaterials
Mechanics of Materials

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10.1016/j.biomaterials.2014.03.011

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@article{be49ec0bea1c4c11b59da90bb3188196,

title = "Carbon nanotube-based substrates for modulation of human pluripotent stem cell fate",

abstract = "We investigated the biological response of human pluripotent stem cells (hPSCs) cultured on a carbon nanotube (CNT) array-based substrate with the long term goal to direct hPSC germ layer specification for a wide variety of tissue engineering applications. CNT arrays were fabricated using a chemical vapor deposition system allowing for control over surface roughness and mechanical stiffness. Our results demonstrated that hPSCs readily attach to hydrophilized and extracellular matrix coated CNT arrays. hPSCs cultured as colonies in conditions supporting self-renewal demonstrated the morphology and marker expression of undifferentiated hPSCs. Conditions inducing spontaneous differentiation lead to hPSC commitment to all three embryonic germ layers as assessed by immunostaining and RT-PCR analysis. Strikingly, the physical characteristics of CNT arrays favored mesodermal specification of hPSCs. This is contradictory to the behavior of hPSCs on traditional tissue culture plastic which promotes the development of ectoderm. Altogether, these results demonstrate the potential of CNT arrays to be used in the generation of new platforms that allow for precise control of hPSC differentiation by tuning the characteristics of their physical microenvironment.",

keywords = "Cell adhesion, Cytoskeleton, Differentiation, Human pluripotent stem cells, Multi-walled carbon nanotubes, Surface roughness",

author = "Pryzhkova, {Marina V.} and Indrat Aria and Qingsu Cheng and Harris, {Greg M.} and Xingjie Zan and Morteza Gharib and Ehsan Jabbarzadeh",

note = "Funding Information: We wish to thank Dr. I. Slukvin and Dr. K. Hu (University of Wisconsin-Madison) for hiPSC lines provided. We also would like to acknowledge Dr. Julia Greer's help with nanoindentation measurements of carbon nanotube arrays. We also would like to acknowledge W. M. Keck Foundation Open Laboratory at USC for the help with AFM measurements. We gratefully acknowledge support from the National Institute of Health (Grant NIH P20 GM103641 ) and National Science Foundation (Grant EPS-0903795 ).",

year = "2014",

month = jun,

doi = "10.1016/j.biomaterials.2014.03.011",

language = "English (US)",

volume = "35",

pages = "5098--5109",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

number = "19",

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TY - JOUR

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AU - Pryzhkova, Marina V.

AU - Aria, Indrat

AU - Cheng, Qingsu

AU - Harris, Greg M.

AU - Zan, Xingjie

AU - Gharib, Morteza

AU - Jabbarzadeh, Ehsan

N1 - Funding Information: We wish to thank Dr. I. Slukvin and Dr. K. Hu (University of Wisconsin-Madison) for hiPSC lines provided. We also would like to acknowledge Dr. Julia Greer's help with nanoindentation measurements of carbon nanotube arrays. We also would like to acknowledge W. M. Keck Foundation Open Laboratory at USC for the help with AFM measurements. We gratefully acknowledge support from the National Institute of Health (Grant NIH P20 GM103641 ) and National Science Foundation (Grant EPS-0903795 ).

PY - 2014/6

Y1 - 2014/6

N2 - We investigated the biological response of human pluripotent stem cells (hPSCs) cultured on a carbon nanotube (CNT) array-based substrate with the long term goal to direct hPSC germ layer specification for a wide variety of tissue engineering applications. CNT arrays were fabricated using a chemical vapor deposition system allowing for control over surface roughness and mechanical stiffness. Our results demonstrated that hPSCs readily attach to hydrophilized and extracellular matrix coated CNT arrays. hPSCs cultured as colonies in conditions supporting self-renewal demonstrated the morphology and marker expression of undifferentiated hPSCs. Conditions inducing spontaneous differentiation lead to hPSC commitment to all three embryonic germ layers as assessed by immunostaining and RT-PCR analysis. Strikingly, the physical characteristics of CNT arrays favored mesodermal specification of hPSCs. This is contradictory to the behavior of hPSCs on traditional tissue culture plastic which promotes the development of ectoderm. Altogether, these results demonstrate the potential of CNT arrays to be used in the generation of new platforms that allow for precise control of hPSC differentiation by tuning the characteristics of their physical microenvironment.

AB - We investigated the biological response of human pluripotent stem cells (hPSCs) cultured on a carbon nanotube (CNT) array-based substrate with the long term goal to direct hPSC germ layer specification for a wide variety of tissue engineering applications. CNT arrays were fabricated using a chemical vapor deposition system allowing for control over surface roughness and mechanical stiffness. Our results demonstrated that hPSCs readily attach to hydrophilized and extracellular matrix coated CNT arrays. hPSCs cultured as colonies in conditions supporting self-renewal demonstrated the morphology and marker expression of undifferentiated hPSCs. Conditions inducing spontaneous differentiation lead to hPSC commitment to all three embryonic germ layers as assessed by immunostaining and RT-PCR analysis. Strikingly, the physical characteristics of CNT arrays favored mesodermal specification of hPSCs. This is contradictory to the behavior of hPSCs on traditional tissue culture plastic which promotes the development of ectoderm. Altogether, these results demonstrate the potential of CNT arrays to be used in the generation of new platforms that allow for precise control of hPSC differentiation by tuning the characteristics of their physical microenvironment.

KW - Cell adhesion

KW - Cytoskeleton

KW - Differentiation

KW - Human pluripotent stem cells

KW - Multi-walled carbon nanotubes

KW - Surface roughness

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Carbon nanotube-based substrates for modulation of human pluripotent stem cell fate

Abstract

Keywords

ASJC Scopus subject areas

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