Carbon Dots as a New Class of Diamagnetic Chemical Exchange Saturation Transfer (diaCEST) MRI Contrast Agents

Jia Zhang, Yue Yuan, Minling Gao, Zheng Han, Chengyan Chu, Yuguo Li, Peter C.M. van Zijl, Mingyao Ying, Jeff W.M. Bulte, Guanshu Liu

Research output: Contribution to journalArticle

Abstract

While carbon dots (C-dots) have been extensively investigated pertaining to their fluorescent, phosphorescent, electrochemiluminescent, optoelectronic, and catalytic features, their inherent chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) properties are unknown. By virtue of their hydrophilicity and abundant exchangeable protons of hydroxyl, amine, and amide anchored on the surface, we report here that C-dots can be adapted as effective diamagnetic CEST (diaCEST) MRI contrast agents. As a proof-of-concept demonstration, human glioma cells were labeled with liposomes with or without encapsulated C-dots and implanted in mouse brain. In vivo CEST MRI was able to clearly differentiate labeled cells from non-labeled cells. The present findings may encourage new applications of C-dots for in vivo imaging in deep tissues, which is currently not possible using conventional fluorescent (near-infrared) C-dots.

Original languageEnglish (US)
JournalAngewandte Chemie - International Edition
DOIs
StatePublished - Jan 1 2019

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Magnetic resonance imaging
Contrast Media
Carbon
Magnetic resonance
Imaging techniques
Liposomes
Hydrophilicity
Amides
Hydroxyl Radical
Optoelectronic devices
Amines
Protons
Brain
Demonstrations
Tissue
Infrared radiation

Keywords

  • carbon dots
  • cell labeling
  • CEST MRI
  • contrast agent
  • intracranial implantation

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

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title = "Carbon Dots as a New Class of Diamagnetic Chemical Exchange Saturation Transfer (diaCEST) MRI Contrast Agents",
abstract = "While carbon dots (C-dots) have been extensively investigated pertaining to their fluorescent, phosphorescent, electrochemiluminescent, optoelectronic, and catalytic features, their inherent chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) properties are unknown. By virtue of their hydrophilicity and abundant exchangeable protons of hydroxyl, amine, and amide anchored on the surface, we report here that C-dots can be adapted as effective diamagnetic CEST (diaCEST) MRI contrast agents. As a proof-of-concept demonstration, human glioma cells were labeled with liposomes with or without encapsulated C-dots and implanted in mouse brain. In vivo CEST MRI was able to clearly differentiate labeled cells from non-labeled cells. The present findings may encourage new applications of C-dots for in vivo imaging in deep tissues, which is currently not possible using conventional fluorescent (near-infrared) C-dots.",
keywords = "carbon dots, cell labeling, CEST MRI, contrast agent, intracranial implantation",
author = "Jia Zhang and Yue Yuan and Minling Gao and Zheng Han and Chengyan Chu and Yuguo Li and {van Zijl}, {Peter C.M.} and Mingyao Ying and Bulte, {Jeff W.M.} and Guanshu Liu",
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T1 - Carbon Dots as a New Class of Diamagnetic Chemical Exchange Saturation Transfer (diaCEST) MRI Contrast Agents

AU - Zhang, Jia

AU - Yuan, Yue

AU - Gao, Minling

AU - Han, Zheng

AU - Chu, Chengyan

AU - Li, Yuguo

AU - van Zijl, Peter C.M.

AU - Ying, Mingyao

AU - Bulte, Jeff W.M.

AU - Liu, Guanshu

PY - 2019/1/1

Y1 - 2019/1/1

N2 - While carbon dots (C-dots) have been extensively investigated pertaining to their fluorescent, phosphorescent, electrochemiluminescent, optoelectronic, and catalytic features, their inherent chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) properties are unknown. By virtue of their hydrophilicity and abundant exchangeable protons of hydroxyl, amine, and amide anchored on the surface, we report here that C-dots can be adapted as effective diamagnetic CEST (diaCEST) MRI contrast agents. As a proof-of-concept demonstration, human glioma cells were labeled with liposomes with or without encapsulated C-dots and implanted in mouse brain. In vivo CEST MRI was able to clearly differentiate labeled cells from non-labeled cells. The present findings may encourage new applications of C-dots for in vivo imaging in deep tissues, which is currently not possible using conventional fluorescent (near-infrared) C-dots.

AB - While carbon dots (C-dots) have been extensively investigated pertaining to their fluorescent, phosphorescent, electrochemiluminescent, optoelectronic, and catalytic features, their inherent chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) properties are unknown. By virtue of their hydrophilicity and abundant exchangeable protons of hydroxyl, amine, and amide anchored on the surface, we report here that C-dots can be adapted as effective diamagnetic CEST (diaCEST) MRI contrast agents. As a proof-of-concept demonstration, human glioma cells were labeled with liposomes with or without encapsulated C-dots and implanted in mouse brain. In vivo CEST MRI was able to clearly differentiate labeled cells from non-labeled cells. The present findings may encourage new applications of C-dots for in vivo imaging in deep tissues, which is currently not possible using conventional fluorescent (near-infrared) C-dots.

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KW - cell labeling

KW - CEST MRI

KW - contrast agent

KW - intracranial implantation

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