Capturing Cardiogenesis in Gastruloids

Giuliana Rossi, Nicolas Broguiere, Matthew Miyamoto, Andrea Boni, Romain Guiet, Mehmet Girgin, Robert G. Kelly, Chulan Kwon, Matthias P. Lutolf

Research output: Contribution to journalArticlepeer-review


Organoids are powerful models for studying tissue development, physiology, and disease. However, current culture systems disrupt the inductive tissue-tissue interactions needed for the complex morphogenetic processes of native organogenesis. Here, we show that mouse embryonic stem cells (mESCs) can be coaxed to robustly undergo fundamental steps of early heart organogenesis with an in-vivo-like spatiotemporal fidelity. These axially patterned embryonic organoids (gastruloids) mimic embryonic development and support the generation of cardiovascular progenitors, including first and second heart fields. The cardiac progenitors self-organize into an anterior domain reminiscent of a cardiac crescent before forming a beating cardiac tissue near a putative primitive gut-like tube, from which it is separated by an endocardial-like layer. These findings unveil the surprising morphogenetic potential of mESCs to execute key aspects of organogenesis through the coordinated development of multiple tissues. This platform could be an excellent tool for studying heart development in unprecedented detail and throughput.

Original languageEnglish (US)
Pages (from-to)230-240.e6
JournalCell stem cell
Issue number2
StatePublished - Feb 4 2021


  • 3D cardiac tissue
  • cardiac organoid
  • cardiogenesis
  • development
  • embryonic organoids
  • heart
  • in vitro organogenesis

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Cell Biology


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