Capsule growth in Cryptococcus neoformans is coordinated with cell cycle progression

Rocío García-Rodas, Radames Cordero-Gonzalez, Nuria Trevijano-Contador, Guilhem Janbon, Frédérique Moyrand, Arturo Casadevall, Oscar Zaragoza

Research output: Contribution to journalArticle

Abstract

The fungal pathogen Cryptococcus neoformans has several virulence factors, among which the most important is a polysaccharide capsule. The size of the capsule is variable and can increase significantly during infection. In this work, we investigated the relationship between capsular enlargement and the cell cycle. Capsule growth occurred primarily during the G1 phase. Real-time visualization of capsule growth demonstrated that this process occurred before the appearance of the bud and that capsule growth arrested during budding. Benomyl, which arrests the cells in G2/M, inhibited capsule growth, while sirolimus (rapamycin) addition, which induces G1 arrest, resulted in cells with larger capsule. Furthermore, we have characterized a mutant strain that lacks a putative G1/S cyclin. This mutant showed an increased capacity to enlarge the capsule, both in vivo (using Galleria mellonella as the host model) and in vitro. In the absence of Cln1, there was a significant increase in the production of extracellular vesicles. Proteomic assays suggest that in the cln1 mutant strain, there is an upregulation of the glyoxylate acid cycle. Besides, this cyclin mutant is avirulent at 37°C, which correlates with growth defects at this temperature in rich medium. In addition, the cln1 mutant showed lower intracellular replication rates in murine macrophages. We conclude that cell cycle regulatory elements are involved in the modulation of the expression of the main virulence factor in C. neoformans.

Original languageEnglish (US)
JournalmBio
Volume5
Issue number3
DOIs
StatePublished - Jun 17 2014
Externally publishedYes

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Cryptococcus neoformans
Capsules
Cell Cycle
Growth
Virulence Factors
Sirolimus
Cyclin G1
Benomyl
Cyclins
G1 Phase
Proteomics
Polysaccharides
Up-Regulation
Macrophages
Temperature
Acids
Infection

ASJC Scopus subject areas

  • Microbiology
  • Virology
  • Medicine(all)

Cite this

Capsule growth in Cryptococcus neoformans is coordinated with cell cycle progression. / García-Rodas, Rocío; Cordero-Gonzalez, Radames; Trevijano-Contador, Nuria; Janbon, Guilhem; Moyrand, Frédérique; Casadevall, Arturo; Zaragoza, Oscar.

In: mBio, Vol. 5, No. 3, 17.06.2014.

Research output: Contribution to journalArticle

García-Rodas, Rocío ; Cordero-Gonzalez, Radames ; Trevijano-Contador, Nuria ; Janbon, Guilhem ; Moyrand, Frédérique ; Casadevall, Arturo ; Zaragoza, Oscar. / Capsule growth in Cryptococcus neoformans is coordinated with cell cycle progression. In: mBio. 2014 ; Vol. 5, No. 3.
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