Capsule enlargement in Cryptococcus neoformans confers resistance to oxidative stress suggesting a mechanism for intracellular survival

Oscar Zaragoza, Cara J. Chrisman, Maria Victoria Castelli, Susana Frases, Manuel Cuenca-Estrella, Juan Luis Rodríguez-Tudela, Arturo Casadevall

Research output: Contribution to journalArticle

Abstract

Cryptococcus neoformans is a facultative intracellular pathogen. The most distinctive feature of C. neoformans is a polysaccharide capsule that enlarges depending on environmental stimuli. The mechanism by which C. neoformans avoids killing during phagocytosis is unknown. We hypothesized that capsule growth conferred resistance to microbicidal molecules produced by the host during infection, particularly during phagocytosis. We observed that capsule enlargement conferred resistance to reactive oxygen species produced by H2O2 that was not associated with a higher catalase activity, suggesting a new function for the capsule as a scavenger of reactive oxidative intermediates. Soluble capsular polysaccharide protected C.neoformans and Saccharomyces cerevisiae from killing by H2O2. Acapsular mutants had higher susceptibility to free radicals. Capsular polysaccharide acted as an antioxidant in the nitroblue tetrazolium (NBT) reduction coupled to β-nicotinamide adenine dinucleotide (NADH)/phenazine methosulfate (PMS) assay. Capsule enlargement conferred resistance to antimicrobial peptides and the antifungal drug Amphotericin B. Interestingly, the capsule had no effect on susceptibility to azoles and increased susceptibility to fluconazole. Capsule enlargement reduced phagocytosis by environmental predators, although we also noticed that in this system, starvation of C.neoformans cells produced resistance to phagocytosis. Our results suggest that capsular enlargement is a mechanism that enhances C. neoformans survival when ingested by phagocytic cells.

Original languageEnglish (US)
Pages (from-to)2043-2057
Number of pages15
JournalCellular Microbiology
Volume10
Issue number10
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Cryptococcus neoformans
Oxidative stress
Capsules
Oxidative Stress
Phagocytosis
Polysaccharides
NAD
Methylphenazonium Methosulfate
Nitroblue Tetrazolium
Azoles
Fluconazole
Amphotericin B
Pathogens
Phagocytes
Starvation
Yeast
Catalase
Free Radicals
Saccharomyces cerevisiae
Assays

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Microbiology

Cite this

Capsule enlargement in Cryptococcus neoformans confers resistance to oxidative stress suggesting a mechanism for intracellular survival. / Zaragoza, Oscar; Chrisman, Cara J.; Castelli, Maria Victoria; Frases, Susana; Cuenca-Estrella, Manuel; Rodríguez-Tudela, Juan Luis; Casadevall, Arturo.

In: Cellular Microbiology, Vol. 10, No. 10, 2008, p. 2043-2057.

Research output: Contribution to journalArticle

Zaragoza, Oscar ; Chrisman, Cara J. ; Castelli, Maria Victoria ; Frases, Susana ; Cuenca-Estrella, Manuel ; Rodríguez-Tudela, Juan Luis ; Casadevall, Arturo. / Capsule enlargement in Cryptococcus neoformans confers resistance to oxidative stress suggesting a mechanism for intracellular survival. In: Cellular Microbiology. 2008 ; Vol. 10, No. 10. pp. 2043-2057.
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