Capsaicin-sensitive and -insensitive vagal bronchopulmonary C-fibres in the mouse

Marian Kollarik, Q. Thai Dinh, Axel Fischer, Bradley J Undem

Research output: Contribution to journalArticle

Abstract

We developed an isolated tracheally perfused (35-37°C) nerve-lung preparation for the study of bronchopulmonary afferent nerve activity in the mouse. Extracellular recordings were made from the vagal sensory neurons located in the jugular-nodose ganglia complex (JNC) with identified receptive fields in the lungs. Analysis of the vagal compound action potential revealed that the mouse vagal C-fibre conduction velocities range from 0.3 to 1.5 m s-1. A total of 83 bronchopulmonary C-fibres were studied. The sensitivity of the bronchopulmonary C-fibres to the vanilloid receptor 1 (VR1) agonist capsaicin was dependent on conduction velocity. Thus C-fibres with conduction velocities between 0.3 and 0.7 m s-1 responded to capsaicin (1 μM) while C-fibres with conduction velocities between 0.7 and 1.5 m s-1 were capsaicin insensitive. Similarly, bradykinin (1 μM) excited only those C-fibres with conduction velocities <0.7 m s-1. The response to bradykinin was not mimicked by the B1 receptor agonist [des-Arg9]bradykinin (1 μM) and was abolished by the bradykinin B2 receptor antagonist HOE 140 (1 μM). Adenosine 5′-triphosphate (ATP, 30 μM) activated the C-fibres irrespective of the conduction velocities. This response was mimicked by the selective P2X agonist α,β-methylene-adenosine 5′-triphosphate (30 μM). Consistent with the electrophysiology, morphological analysis revealed that only ∼40% of the lung-specific small diameter (<20 μM) JNC neurons consistent with the C-fibre cell bodies display VR1 immunoreactivity. This study describes a convenient in vitro method for the study of mouse bronchopulmonary C-fibres. The results indicate that C-fibres in the mouse lungs are not homogeneous, but can be subclassified into capsaicin-sensitive and capsaicin-insensitive phenotypes.

Original languageEnglish (US)
Pages (from-to)869-879
Number of pages11
JournalJournal of Physiology
Volume551
Issue number3
DOIs
StatePublished - Sep 15 2003

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Unmyelinated Nerve Fibers
Capsaicin
Bradykinin
Nodose Ganglion
Lung
Adenosine Triphosphate
Neck
Electrophysiology
Sensory Receptor Cells
Action Potentials
Phenotype
Neurons

ASJC Scopus subject areas

  • Physiology

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Capsaicin-sensitive and -insensitive vagal bronchopulmonary C-fibres in the mouse. / Kollarik, Marian; Dinh, Q. Thai; Fischer, Axel; Undem, Bradley J.

In: Journal of Physiology, Vol. 551, No. 3, 15.09.2003, p. 869-879.

Research output: Contribution to journalArticle

Kollarik, Marian ; Dinh, Q. Thai ; Fischer, Axel ; Undem, Bradley J. / Capsaicin-sensitive and -insensitive vagal bronchopulmonary C-fibres in the mouse. In: Journal of Physiology. 2003 ; Vol. 551, No. 3. pp. 869-879.
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