CAPS and syntaxin dock dense core vesicles to the plasma membrane in neurons

Marc Hammarlund, Shigeki Watanabe, Kim Schuske, Erik M. Jorgensen

Research output: Contribution to journalArticle

Abstract

Docking to the plasma membrane prepares vesicles for rapid release. Here, we describe a mechanism for dense core vesicle docking in neurons. In Caenorhabditis elegans motor neurons, dense core vesicles dock at the plasma membrane but are excluded from active zones at synapses. We have found that the calcium-activated protein for secretion (CAPS) protein is required for dense core vesicle docking but not synaptic vesicle docking. In contrast, we see that UNC-13, a docking factor for synaptic vesicles, is not essential for dense core vesicle docking. Both the CAPS and UNC-13 docking pathways converge on syntaxin, a component of the SNARE (soluble N-ethyl-maleimide-sensitive fusion protein attachment receptor) complex. Overexpression of open syntaxin can bypass the requirement for CAPS in dense core vesicle docking. Thus, CAPS likely promotes the open state of syntaxin, which then docks dense core vesicles. CAPS function in dense core vesicle docking parallels UNC-13 in synaptic vesicle docking, which suggests that these related proteins act similarly to promote docking of independent vesicle populations.

Original languageEnglish (US)
Pages (from-to)483-491
Number of pages9
JournalJournal of Cell Biology
Volume180
Issue number3
DOIs
StatePublished - Feb 11 2008
Externally publishedYes

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Qa-SNARE Proteins
Secretory Vesicles
Cell Membrane
Calcium
Neurons
Synaptic Vesicles
Proteins
SNARE Proteins
Caenorhabditis elegans
Motor Neurons
Synapses

ASJC Scopus subject areas

  • Cell Biology

Cite this

CAPS and syntaxin dock dense core vesicles to the plasma membrane in neurons. / Hammarlund, Marc; Watanabe, Shigeki; Schuske, Kim; Jorgensen, Erik M.

In: Journal of Cell Biology, Vol. 180, No. 3, 11.02.2008, p. 483-491.

Research output: Contribution to journalArticle

Hammarlund, Marc ; Watanabe, Shigeki ; Schuske, Kim ; Jorgensen, Erik M. / CAPS and syntaxin dock dense core vesicles to the plasma membrane in neurons. In: Journal of Cell Biology. 2008 ; Vol. 180, No. 3. pp. 483-491.
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