Capillary and venule proliferation in the healing process of Dacron venous grafts in rats

Hai Zhang, G. Melville Williams

Research output: Contribution to journalArticle

Abstract

A woven, noncrimped graft (made of Dacron and coated with an elastomer) that was 1.5 mm in diameter and 15 mm long was capable of replacing the vena cava in rats. The elastomer mixture consisting mainly of silicone rubber was necessary to bond the woven Dacron fibrils of the woven graft to prevent fraying at the anastomoses and bleeding through the interstices of the lightly woven, fairly high porosity (500) conduit. Sequential histologic studies showed that patency was associated with the ingrowth of small venules and the spreading of endothelial cells from each anastomosis toward the center. Small venules appeared in loose connective tissue, forming a pseudointima 12 days after grafting. This process occurred in the midportion of the graft before pannus endothelial growth covered the endothelial surface. Various thicknesses of the external polymer coat were studied for their influence on healing. None of the grafts developed a thrombus in these later studies, and regardless of the thickness of the external elastomer, endothelial resurfacing was complete at 30 days. However, the graft with the thinnest external elastomer coating had the best-formed vasa vasorum, and the intima at both the anastomoses and midportions of the graft was significantly thinner than intima found in grafts of other composition. We conclude that this woven Dacron polygraft provides a surface resistant to early thrombosis; that healing occurs mainly by pannus ingrowth, but external and interstitial factors are also important; and that properties inherent in the polygraft wall determine the size of the residual lumen.

Original languageEnglish (US)
Pages (from-to)409-415
Number of pages7
JournalSurgery
Volume111
Issue number4
StatePublished - 1992

Fingerprint

Polyethylene Terephthalates
Venules
Elastomers
Transplants
Thrombosis
Vasa Vasorum
Silicone Elastomers
Venae Cavae
Porosity
Connective Tissue
Polymers
Endothelial Cells
Hemorrhage
Growth

ASJC Scopus subject areas

  • Surgery

Cite this

Capillary and venule proliferation in the healing process of Dacron venous grafts in rats. / Zhang, Hai; Williams, G. Melville.

In: Surgery, Vol. 111, No. 4, 1992, p. 409-415.

Research output: Contribution to journalArticle

Zhang, H & Williams, GM 1992, 'Capillary and venule proliferation in the healing process of Dacron venous grafts in rats', Surgery, vol. 111, no. 4, pp. 409-415.
Zhang, Hai ; Williams, G. Melville. / Capillary and venule proliferation in the healing process of Dacron venous grafts in rats. In: Surgery. 1992 ; Vol. 111, No. 4. pp. 409-415.
@article{204e30ed61dd46b5ac338a0186e8b1e0,
title = "Capillary and venule proliferation in the healing process of Dacron venous grafts in rats",
abstract = "A woven, noncrimped graft (made of Dacron and coated with an elastomer) that was 1.5 mm in diameter and 15 mm long was capable of replacing the vena cava in rats. The elastomer mixture consisting mainly of silicone rubber was necessary to bond the woven Dacron fibrils of the woven graft to prevent fraying at the anastomoses and bleeding through the interstices of the lightly woven, fairly high porosity (500) conduit. Sequential histologic studies showed that patency was associated with the ingrowth of small venules and the spreading of endothelial cells from each anastomosis toward the center. Small venules appeared in loose connective tissue, forming a pseudointima 12 days after grafting. This process occurred in the midportion of the graft before pannus endothelial growth covered the endothelial surface. Various thicknesses of the external polymer coat were studied for their influence on healing. None of the grafts developed a thrombus in these later studies, and regardless of the thickness of the external elastomer, endothelial resurfacing was complete at 30 days. However, the graft with the thinnest external elastomer coating had the best-formed vasa vasorum, and the intima at both the anastomoses and midportions of the graft was significantly thinner than intima found in grafts of other composition. We conclude that this woven Dacron polygraft provides a surface resistant to early thrombosis; that healing occurs mainly by pannus ingrowth, but external and interstitial factors are also important; and that properties inherent in the polygraft wall determine the size of the residual lumen.",
author = "Hai Zhang and Williams, {G. Melville}",
year = "1992",
language = "English (US)",
volume = "111",
pages = "409--415",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "4",

}

TY - JOUR

T1 - Capillary and venule proliferation in the healing process of Dacron venous grafts in rats

AU - Zhang, Hai

AU - Williams, G. Melville

PY - 1992

Y1 - 1992

N2 - A woven, noncrimped graft (made of Dacron and coated with an elastomer) that was 1.5 mm in diameter and 15 mm long was capable of replacing the vena cava in rats. The elastomer mixture consisting mainly of silicone rubber was necessary to bond the woven Dacron fibrils of the woven graft to prevent fraying at the anastomoses and bleeding through the interstices of the lightly woven, fairly high porosity (500) conduit. Sequential histologic studies showed that patency was associated with the ingrowth of small venules and the spreading of endothelial cells from each anastomosis toward the center. Small venules appeared in loose connective tissue, forming a pseudointima 12 days after grafting. This process occurred in the midportion of the graft before pannus endothelial growth covered the endothelial surface. Various thicknesses of the external polymer coat were studied for their influence on healing. None of the grafts developed a thrombus in these later studies, and regardless of the thickness of the external elastomer, endothelial resurfacing was complete at 30 days. However, the graft with the thinnest external elastomer coating had the best-formed vasa vasorum, and the intima at both the anastomoses and midportions of the graft was significantly thinner than intima found in grafts of other composition. We conclude that this woven Dacron polygraft provides a surface resistant to early thrombosis; that healing occurs mainly by pannus ingrowth, but external and interstitial factors are also important; and that properties inherent in the polygraft wall determine the size of the residual lumen.

AB - A woven, noncrimped graft (made of Dacron and coated with an elastomer) that was 1.5 mm in diameter and 15 mm long was capable of replacing the vena cava in rats. The elastomer mixture consisting mainly of silicone rubber was necessary to bond the woven Dacron fibrils of the woven graft to prevent fraying at the anastomoses and bleeding through the interstices of the lightly woven, fairly high porosity (500) conduit. Sequential histologic studies showed that patency was associated with the ingrowth of small venules and the spreading of endothelial cells from each anastomosis toward the center. Small venules appeared in loose connective tissue, forming a pseudointima 12 days after grafting. This process occurred in the midportion of the graft before pannus endothelial growth covered the endothelial surface. Various thicknesses of the external polymer coat were studied for their influence on healing. None of the grafts developed a thrombus in these later studies, and regardless of the thickness of the external elastomer, endothelial resurfacing was complete at 30 days. However, the graft with the thinnest external elastomer coating had the best-formed vasa vasorum, and the intima at both the anastomoses and midportions of the graft was significantly thinner than intima found in grafts of other composition. We conclude that this woven Dacron polygraft provides a surface resistant to early thrombosis; that healing occurs mainly by pannus ingrowth, but external and interstitial factors are also important; and that properties inherent in the polygraft wall determine the size of the residual lumen.

UR - http://www.scopus.com/inward/record.url?scp=0026704250&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026704250&partnerID=8YFLogxK

M3 - Article

C2 - 1373008

AN - SCOPUS:0026704250

VL - 111

SP - 409

EP - 415

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 4

ER -