Capacitative ca2+ entry in agonist-induced pulmonary vasoconstriction

Sharon S. Mcdaniel, Oleksandr Platoshyn, Jian Wang, Ying Yu, Michele Sweeney, Stefanie Krick, Lewis J. Rubin, Jason X J Yuan

Research output: Contribution to journalArticle

Abstract

Agonist-induced increases in cytosolic Ca2+ concentration ([Ca2+]cyt) in pulmonary artery (PA) smooth muscle cells (SMCs) consist of a transient Ca2+ release from intracellular stores followed by a sustained Ca2+ influx. Depletion of intracellular Ca2+ stores triggers capacitative Ca2+ entry (CCE), which contributes to the sustained increase in [Ca2+]cyt and the refilling of Ca2+ into the stores. In isolated PAs superfused with Ca2+-free solution, phenylephrine induced a transient contraction, apparently by a rise in [Ca2+]cyt due to Ca2+ release from the intracellular stores. The transient contraction lasted for 3-4 min until the Ca2+ store was depleted. Restoration of extracellular Ca2+ in the presence of phentolamine produced a contraction potentially due to a rise in [Ca2+]cyt via CCE. The store-operated Ca2+ channel blocker Ni2+ reduced the store depletion-activated Ca2+ currents, decreased CCE, and inhibited the CCE-mediated contraction. In single PASMCs, we identified, using RT-PCR, five transient receptor potential gene transcripts. These results suggest that CCE, potentially through transient receptor potential-encoded Ca2+ channels, plays an important role in agonist-mediated PA contraction.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume280
Issue number5 25-5
StatePublished - May 2001
Externally publishedYes

Fingerprint

Vasoconstriction
Pulmonary Artery
Lung
Phentolamine
Phenylephrine
Smooth Muscle Myocytes
Polymerase Chain Reaction
Genes

Keywords

  • Pulmonary artery smooth muscle cells
  • Pulmonary hypertension
  • Transient receptor potential gene

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Mcdaniel, S. S., Platoshyn, O., Wang, J., Yu, Y., Sweeney, M., Krick, S., ... Yuan, J. X. J. (2001). Capacitative ca2+ entry in agonist-induced pulmonary vasoconstriction. American Journal of Physiology - Lung Cellular and Molecular Physiology, 280(5 25-5).

Capacitative ca2+ entry in agonist-induced pulmonary vasoconstriction. / Mcdaniel, Sharon S.; Platoshyn, Oleksandr; Wang, Jian; Yu, Ying; Sweeney, Michele; Krick, Stefanie; Rubin, Lewis J.; Yuan, Jason X J.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 280, No. 5 25-5, 05.2001.

Research output: Contribution to journalArticle

Mcdaniel, SS, Platoshyn, O, Wang, J, Yu, Y, Sweeney, M, Krick, S, Rubin, LJ & Yuan, JXJ 2001, 'Capacitative ca2+ entry in agonist-induced pulmonary vasoconstriction', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 280, no. 5 25-5.
Mcdaniel, Sharon S. ; Platoshyn, Oleksandr ; Wang, Jian ; Yu, Ying ; Sweeney, Michele ; Krick, Stefanie ; Rubin, Lewis J. ; Yuan, Jason X J. / Capacitative ca2+ entry in agonist-induced pulmonary vasoconstriction. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2001 ; Vol. 280, No. 5 25-5.
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