Canine GM2 gangliosidosis: morphological and biochemical analysis.

Harvey Singer, L. C. Cork

Research output: Contribution to journalArticle

Abstract

Beta-hexosaminidase activity and the effects of ganglioside storage on neuronal function were examined in a German shorthair pointer (GSHP) with progressive neurodegenerative signs. Morphologic evidence of neuronal storage and massive accumulation of GM2 ganglioside were present. Beta-hexosaminidase activity in plasma, liver, kidney, and brain, assayed with use of unsulfated fluorogenic substrates, was normal. There was no pathologic accumulation or aberrant localization of phosphorylated neurofilaments in neurons. Activity of cortical neurotransmitter synthesizing enzymes, choline acetyltransferase, and glutamate decarboxylase was unaffected. Ligand binding to carrier sites for choline high affinity uptake identified with [3H]hemicholinium-3 was increased, whereas post-synaptic binding to muscarinic cholinergic ([3H]QNB) and gamma-aminobutyric acid receptors ([3H]muscimol) was reduced.

Original languageEnglish (US)
Pages (from-to)114-120
Number of pages7
JournalVeterinary Pathology
Volume26
Issue number2
StatePublished - Mar 1989

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GM2 Gangliosidosis
beta-N-Acetylhexosaminidases
beta-N-acetylhexosaminidase
gangliosides
Cholinergic Agents
Canidae
G(M2) Ganglioside
Hemicholinium 3
glutamate decarboxylase
choline acetyltransferase
Muscimol
GABA Receptors
Glutamate Decarboxylase
Choline O-Acetyltransferase
Intermediate Filaments
dogs
Gangliosides
cholinergic agents
choline
neurotransmitters

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Canine GM2 gangliosidosis : morphological and biochemical analysis. / Singer, Harvey; Cork, L. C.

In: Veterinary Pathology, Vol. 26, No. 2, 03.1989, p. 114-120.

Research output: Contribution to journalArticle

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