Cancer Protrusions on a Tightrope: Nanofiber Curvature Contrast Quantitates Single Protrusion Dynamics

Brian Koons, Puja Sharma, Zhou Ye, Apratim Mukherjee, Meng Horng Lee, Denis Wirtz, Bahareh Behkam, Amrinder S. Nain

Research output: Contribution to journalArticle

Abstract

Cell migration is studied with the traditional focus on protrusion-driven cell body displacement, while less is known on morphodynamics of individual protrusions themselves, especially in fibrous environments mimicking extracellular matrix. Here, using suspended fibers, we report integrative and multiscale abilities to study protrusive behavior independent of cell body migration. By manipulating the diameter of fibers in orthogonal directions, we constrain cell migration along large diameter (2 μm) base fibers, while solely allowing cells to sense, initiate, and mature protrusions on orthogonally deposited high-curvature/low diameter (∼100, 200, and 600 nm) protrusive fibers and low-curvature (∼300 and 600 nm width) protrusive flat ribbons. In doing so, we report a set of morphodynamic metrics that precisely quantitate protrusion dynamics. Protrusion growth and maturation occur by rapid broadening at the base to achieve long lengths, a behavior dramatically influenced by curvature. While flat ribbons universally induce the formation of broad and long protrusions, we quantitatively protrutype protrusive behavior of two highly invasive cancer cell lines and find breast adenocarcinoma (MDA-MB-231) to exhibit sensitivity to fiber curvature higher than that of brain glioblastoma DBTRG-05MG. Furthermore, while actin and microtubules localize within protrusions of all sizes, we quantify protrusion size-driven localization of vimentin and, contrary to current understanding, report that vimentin is not required to form protrusions. Using multiple protrusive fibers, we quantify high coordination between hierarchical branches of individual protrusions and describe how the spatial configuration of multiple protrusions regulates cell migratory state. Finally, we describe protrusion-driven shedding and collection of cytoplasmic debris.

Original languageEnglish (US)
Pages (from-to)12037-12048
Number of pages12
JournalACS Nano
Volume11
Issue number12
DOIs
StatePublished - Dec 26 2017

Fingerprint

Nanofibers
cancer
curvature
fibers
Fibers
Cells
Vimentin
cells
ribbons
multiple docking adapters
cultured cells
debris
Debris
breast
brain
Actins
Brain
sensitivity
matrices
configurations

Keywords

  • aligned fibers
  • cell debris
  • cell migration
  • fiber curvature
  • nanofibers
  • protrusion branching
  • protrusions
  • vimentin

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Koons, B., Sharma, P., Ye, Z., Mukherjee, A., Lee, M. H., Wirtz, D., ... Nain, A. S. (2017). Cancer Protrusions on a Tightrope: Nanofiber Curvature Contrast Quantitates Single Protrusion Dynamics. ACS Nano, 11(12), 12037-12048. https://doi.org/10.1021/acsnano.7b04567

Cancer Protrusions on a Tightrope : Nanofiber Curvature Contrast Quantitates Single Protrusion Dynamics. / Koons, Brian; Sharma, Puja; Ye, Zhou; Mukherjee, Apratim; Lee, Meng Horng; Wirtz, Denis; Behkam, Bahareh; Nain, Amrinder S.

In: ACS Nano, Vol. 11, No. 12, 26.12.2017, p. 12037-12048.

Research output: Contribution to journalArticle

Koons, B, Sharma, P, Ye, Z, Mukherjee, A, Lee, MH, Wirtz, D, Behkam, B & Nain, AS 2017, 'Cancer Protrusions on a Tightrope: Nanofiber Curvature Contrast Quantitates Single Protrusion Dynamics', ACS Nano, vol. 11, no. 12, pp. 12037-12048. https://doi.org/10.1021/acsnano.7b04567
Koons, Brian ; Sharma, Puja ; Ye, Zhou ; Mukherjee, Apratim ; Lee, Meng Horng ; Wirtz, Denis ; Behkam, Bahareh ; Nain, Amrinder S. / Cancer Protrusions on a Tightrope : Nanofiber Curvature Contrast Quantitates Single Protrusion Dynamics. In: ACS Nano. 2017 ; Vol. 11, No. 12. pp. 12037-12048.
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