TY - JOUR
T1 - Cancer cells display increased migration and deformability in pace with metastatic progression
AU - Liu, Zhenhui
AU - Lee, Se Jong
AU - Park, Seungman
AU - Konstantopoulos, Konstantinos
AU - Glunde, Kristine
AU - Chen, Yun
AU - Barman, Ishan
N1 - Funding Information:
Z.L. and I.B. acknowledge the support from the National Institute of Biomedical Imaging and Bioengineering (2‐P41‐EB015871‐31) and National Institute of General Medical Sciences (DP2GM128198). S.L. and K.K. acknowledge the support of the National Cancer Institute (R01 CA183804). K.G. acknowledges the support of the National Cancer Institute (R01 CA213428, R01 CA213492). S.P. and Y.C. acknowledge the support from the Maryland Stem Cell Research Fund (2018‐MSCRFF‐4276). We thank Dr. Richard Superfine for the generous gift of the 3D magnetic tweezers system, and the technical assistance offered by Drs. E. Timothy O’Brien and Jeremy Cribb at the University of North Carolina at Chapel Hill.
Publisher Copyright:
© 2020 Federation of American Societies for Experimental Biology
PY - 2020/7/1
Y1 - 2020/7/1
N2 - In this study, we explored the relation between metastatic states vs the capacity of confined migration, amoeboid transition, and cellular stiffness. We compared across an isogenic panel of human breast cancer cells derived from MDA-MB-231 cells. It was observed that cells after lung metastasis have the fastest migration and lowest stiffness, with a significantly higher capacity to transition into an amoeboid mode. Our findings illustrate that metastasis is a selective process favoring motile and softer cells. Moreover, the observation that circulating tumor cells resemble the parental cell line, but not lung-metastatic cells, suggests that cells with higher deformability and motility are likely selected during extravasation and colonization.
AB - In this study, we explored the relation between metastatic states vs the capacity of confined migration, amoeboid transition, and cellular stiffness. We compared across an isogenic panel of human breast cancer cells derived from MDA-MB-231 cells. It was observed that cells after lung metastasis have the fastest migration and lowest stiffness, with a significantly higher capacity to transition into an amoeboid mode. Our findings illustrate that metastasis is a selective process favoring motile and softer cells. Moreover, the observation that circulating tumor cells resemble the parental cell line, but not lung-metastatic cells, suggests that cells with higher deformability and motility are likely selected during extravasation and colonization.
KW - amoeboid movement
KW - breast cancer metastasis
KW - isogenic cell lines
KW - mechanotyping
KW - microchannel migration
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U2 - 10.1096/fj.202000101RR
DO - 10.1096/fj.202000101RR
M3 - Article
C2 - 32463148
AN - SCOPUS:85085584875
VL - 34
SP - 9307
EP - 9315
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 7
ER -