Cancer-associated mutations in endometriosis without cancer

M. S. Anglesio, N. Papadopoulos, A. Ayhan, T. M. Nazeran, M. Noë, H. M. Horlings, A. Lum, S. Jones, J. Senz, T. Seckin, J. Ho, R. C. Wu, V. Lac, H. Ogawa, B. Tessier-Cloutier, R. Alhassan, A. Wang, Y. Wang, J. D. Cohen, F. WongA. Hasanovic, N. Orr, M. Zhang, M. Popoli, W. McMahon, L. D. Wood, A. Mattox, C. Allaire, J. Segars, C. Williams, C. Tomasetti, N. Boyd, K. W. Kinzler, C. B. Gilks, L. Diaz, T. L. Wang, B. Vogelstein, P. J. Yong, D. G. Huntsman, I. M. Shih

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Abstract

BACKGROUND Endometriosis, defined as the presence of ectopic endometrial stroma and epithelium, affects approximately 10% of reproductive-age women and can cause pelvic pain and infertility. Endometriotic lesions are considered to be benign inflammatory lesions but have cancerlike features such as local invasion and resistance to apoptosis. METHODS We analyzed deeply infiltrating endometriotic lesions from 27 patients by means of exomewide sequencing (24 patients) or cancer-driver targeted sequencing (3 patients). Mutations were validated with the use of digital genomic methods in microdissected epithelium and stroma. Epithelial and stromal components of lesions from an additional 12 patients were analyzed by means of a droplet digital polymerase-chain-reaction (PCR) assay for recurrent activating KRAS mutations. RESULTS Exome sequencing revealed somatic mutations in 19 of 24 patients (79%). Five patients harbored known cancer driver mutations in ARID1A, PIK3CA, KRAS, or PPP2R1A, which were validated by Safe-Sequencing System or immunohistochemical analysis. The likelihood of driver genes being affected at this rate in the absence of selection was estimated at P = 0.001 (binomial test). Targeted sequencing and a droplet digital PCR assay identified KRAS mutations in 2 of 3 patients and 3 of 12 patients, respectively, with mutations in the epithelium but not the stroma. One patient harbored two different KRAS mutations, c.35G→T and c.35G→C, and another carried identical KRAS c.35G→A mutations in three distinct lesions. CONCLUSIONS We found that lesions in deep infiltrating endometriosis, which are associated with virtually no risk of malignant transformation, harbor somatic cancer driver mutations. Ten of 39 deep infiltrating lesions (26%) carried driver mutations; all the tested somatic mutations appeared to be confined to the epithelial compartment of endometriotic lesions.

Original languageEnglish (US)
Pages (from-to)1835-1848
Number of pages14
JournalNew England Journal of Medicine
Volume376
Issue number19
DOIs
StatePublished - May 11 2017

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ASJC Scopus subject areas

  • Medicine(all)

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Anglesio, M. S., Papadopoulos, N., Ayhan, A., Nazeran, T. M., Noë, M., Horlings, H. M., Lum, A., Jones, S., Senz, J., Seckin, T., Ho, J., Wu, R. C., Lac, V., Ogawa, H., Tessier-Cloutier, B., Alhassan, R., Wang, A., Wang, Y., Cohen, J. D., ... Shih, I. M. (2017). Cancer-associated mutations in endometriosis without cancer. New England Journal of Medicine, 376(19), 1835-1848. https://doi.org/10.1056/NEJMoa1614814