CaMV 35S promoter directs β-glucuronidase expression in Ganoderma lucidum and Pleurotus citrinopileatus

Li Sun, Huaqing Cai, Weihong Xu, Yuanlei Hu, Zhongping Lin

Research output: Contribution to journalArticlepeer-review

Abstract

The cauliflower mosaic virus (CaMV) 35S promoter has been most commonly used in plant transformation studies, but its activity in mushrooms has not been reported, p301-b is a binary vector containing a bialaphos resistance gene driven by the promoter of Lentinus edodes glyceraldehyde-3-phosphate dehydrogenase (GPD) gene. CaMV 35S-GUS was inserted into p301-b, and the resulting construct p301-bG was transformed to protoplasts of Ganoderma lucidum and basidiospores of Pleurotus citrinopileatus. GUS activity was observed in the transformants, indicating that CaMV 35S promoter can direct expression of exogenous gene in the mushrooms. This is the first report on the application of CaMV 35S promoter in genetic modification of mushrooms.

Original languageEnglish (US)
Pages (from-to)239-244
Number of pages6
JournalApplied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Volume20
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • CaMV 35S promoter
  • Ganoderma lucidum
  • Glyceraldehyde-3-phosphate dehydrogenase
  • Lentinus edodes
  • Pleurotus citrinopileatus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

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