CaMV 35S promoter directs β-glucuronidase expression in Ganoderma lucidum and Pleurotus citrinopileatus

Li Sun; Huaqing Cai; Weihong Xu; Yuanlei Hu; Zhongping Lin

doi:10.1385/MB:20:3:239

CaMV 35S promoter directs β-glucuronidase expression in Ganoderma lucidum and Pleurotus citrinopileatus

Li Sun, Huaqing Cai, Weihong Xu, Yuanlei Hu, Zhongping Lin

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The cauliflower mosaic virus (CaMV) 35S promoter has been most commonly used in plant transformation studies, but its activity in mushrooms has not been reported, p301-b is a binary vector containing a bialaphos resistance gene driven by the promoter of Lentinus edodes glyceraldehyde-3-phosphate dehydrogenase (GPD) gene. CaMV 35S-GUS was inserted into p301-b, and the resulting construct p301-bG was transformed to protoplasts of Ganoderma lucidum and basidiospores of Pleurotus citrinopileatus. GUS activity was observed in the transformants, indicating that CaMV 35S promoter can direct expression of exogenous gene in the mushrooms. This is the first report on the application of CaMV 35S promoter in genetic modification of mushrooms.

Original language	English (US)
Pages (from-to)	239-244
Number of pages	6
Journal	Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Volume	20
Issue number	3
DOIs	https://doi.org/10.1385/MB:20:3:239
State	Published - 2002
Externally published	Yes

Keywords

CaMV 35S promoter
Ganoderma lucidum
Glyceraldehyde-3-phosphate dehydrogenase
Lentinus edodes
Pleurotus citrinopileatus

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Biochemistry
Biotechnology
Applied Microbiology and Biotechnology
Bioengineering

Access to Document

10.1385/MB:20:3:239

Cite this

@article{d0ecff838699407e9f9405b6081a842b,

title = "CaMV 35S promoter directs β-glucuronidase expression in Ganoderma lucidum and Pleurotus citrinopileatus",

abstract = "The cauliflower mosaic virus (CaMV) 35S promoter has been most commonly used in plant transformation studies, but its activity in mushrooms has not been reported, p301-b is a binary vector containing a bialaphos resistance gene driven by the promoter of Lentinus edodes glyceraldehyde-3-phosphate dehydrogenase (GPD) gene. CaMV 35S-GUS was inserted into p301-b, and the resulting construct p301-bG was transformed to protoplasts of Ganoderma lucidum and basidiospores of Pleurotus citrinopileatus. GUS activity was observed in the transformants, indicating that CaMV 35S promoter can direct expression of exogenous gene in the mushrooms. This is the first report on the application of CaMV 35S promoter in genetic modification of mushrooms.",

keywords = "CaMV 35S promoter, Ganoderma lucidum, Glyceraldehyde-3-phosphate dehydrogenase, Lentinus edodes, Pleurotus citrinopileatus",

author = "Li Sun and Huaqing Cai and Weihong Xu and Yuanlei Hu and Zhongping Lin",

year = "2002",

doi = "10.1385/MB:20:3:239",

language = "English (US)",

volume = "20",

pages = "239--244",

journal = "Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology",

issn = "1073-6085",

publisher = "Humana Press",

number = "3",

}

TY - JOUR

T1 - CaMV 35S promoter directs β-glucuronidase expression in Ganoderma lucidum and Pleurotus citrinopileatus

AU - Sun, Li

AU - Cai, Huaqing

AU - Xu, Weihong

AU - Hu, Yuanlei

AU - Lin, Zhongping

PY - 2002

Y1 - 2002

N2 - The cauliflower mosaic virus (CaMV) 35S promoter has been most commonly used in plant transformation studies, but its activity in mushrooms has not been reported, p301-b is a binary vector containing a bialaphos resistance gene driven by the promoter of Lentinus edodes glyceraldehyde-3-phosphate dehydrogenase (GPD) gene. CaMV 35S-GUS was inserted into p301-b, and the resulting construct p301-bG was transformed to protoplasts of Ganoderma lucidum and basidiospores of Pleurotus citrinopileatus. GUS activity was observed in the transformants, indicating that CaMV 35S promoter can direct expression of exogenous gene in the mushrooms. This is the first report on the application of CaMV 35S promoter in genetic modification of mushrooms.

AB - The cauliflower mosaic virus (CaMV) 35S promoter has been most commonly used in plant transformation studies, but its activity in mushrooms has not been reported, p301-b is a binary vector containing a bialaphos resistance gene driven by the promoter of Lentinus edodes glyceraldehyde-3-phosphate dehydrogenase (GPD) gene. CaMV 35S-GUS was inserted into p301-b, and the resulting construct p301-bG was transformed to protoplasts of Ganoderma lucidum and basidiospores of Pleurotus citrinopileatus. GUS activity was observed in the transformants, indicating that CaMV 35S promoter can direct expression of exogenous gene in the mushrooms. This is the first report on the application of CaMV 35S promoter in genetic modification of mushrooms.

KW - CaMV 35S promoter

KW - Ganoderma lucidum

KW - Glyceraldehyde-3-phosphate dehydrogenase

KW - Lentinus edodes

KW - Pleurotus citrinopileatus

UR - http://www.scopus.com/inward/record.url?scp=0036201087&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036201087&partnerID=8YFLogxK

U2 - 10.1385/MB:20:3:239

DO - 10.1385/MB:20:3:239

M3 - Article

C2 - 11936254

AN - SCOPUS:0036201087

SN - 1073-6085

VL - 20

SP - 239

EP - 244

JO - Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

JF - Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

IS - 3

ER -

CaMV 35S promoter directs β-glucuronidase expression in Ganoderma lucidum and Pleurotus citrinopileatus

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this