Campylobacter jejuni cytolethal distending toxin mediates release of interleukin-8 from intestinal epithelial cells

Thomas E. Hickey, Annette L. McVeigh, Daniel A. Scott, Ronda E. Michielutti, Alyssa Bixby, Shannon A. Carroll, A. Louis Bourgeois, Patricia Guerry

Research output: Contribution to journalArticlepeer-review

Abstract

Live cells of Campylobacter jejuni and Campylobacter coli can induce release of interleukin-8 (IL-8) from INT407 cells. Additionally, membrane fractions of C. jejuni 81-176, but not membrane fractions of C. coli strains, can also induce release of IL-8. Membrane preparations from 81-176 mutants defective in any of the three membrane-associated protein subunits of cytolethal distending toxin (CDT) were unable to induce IL-8. The presence of the three cdt genes on a shuttle plasmid in trans restored both CDT activity and the ability to release IL-8 to membrane fractions. However, CDT mutations did not affect the ability of 81-176 to induce IL-8 during adherence to or invasion of INT407 cells. When C. jejuni cdt genes were transferred on a shuttle plasmid into a C. coli strain lacking CDT, membrane preparations became positive in both CDT and IL-8 assays. Growth of C. jejuni in physiological levels of sodium deoxycholate released all three CDT proteins, as well as CDT activity and IL-8 activity, from membranes into supernatants. Antibodies against recombinant forms of each of the three CDT subunit proteins neutralized both CDT activity and the activity responsible for IL-8 release. The data suggest that C. jejuni can induce IL-8 release from INT407 cells by two independent mechanisms, one of which requires adherence and/or invasion and the second of which requires CDT.

Original languageEnglish (US)
Pages (from-to)6535-6541
Number of pages7
JournalInfection and immunity
Volume68
Issue number12
DOIs
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Campylobacter jejuni cytolethal distending toxin mediates release of interleukin-8 from intestinal epithelial cells'. Together they form a unique fingerprint.

Cite this