CAMP inhibits migration, ruffling and paxillin accumulation in focal adhesions of pancreatic ductal adenocarcinoma cells: Effects of PKA and EPAC

Alex Burdyga, Alan Conant, Lee Haynes, Jin Zhang, Kees Jalink, Robert Sutton, John Neoptolemos, Eithne Costello, Alexei Tepikin

Research output: Contribution to journalArticle

Abstract

We demonstrated that increasing intracellular cAMP concentrations result in the inhibition of migration of PANC-1 and other pancreatic ductal adenocarcinoma (PDAC) cell types. The rise of cAMP was accompanied by rapid and reversible cessation of ruffling, by inhibition of focal adhesion turnover and by prominent loss of paxillin from focal adhesions. All these phenomena develop rapidly suggesting that cAMP effectors have a direct influence on the cellular migratory apparatus. The role of two primary cAMP effectors, exchange protein activated by cAMP (EPAC) and protein kinase A (PKA), in cAMP-mediated inhibition of PDAC cell migration and migration-associated processes was investigated. Experiments with selective activators of EPAC and PKA demonstrated that the inhibitory effect of cAMP on migration, ruffling, focal adhesion dynamics and paxillin localisation is mediated by PKA, whilst EPAC potentiates migration.

Original languageEnglish (US)
Pages (from-to)2664-2672
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1833
Issue number12
DOIs
StatePublished - Dec 2013

Keywords

  • CAMP
  • Cell migration
  • EPAC
  • Pancreatic ductal adenocarcinoma
  • Paxillin
  • PKA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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