TY - JOUR
T1 - Calpain expression and infiltration of activated T cells in experimental allergic encephalomyelitis over time
T2 - Increased calpain activity begins with onset of disease
AU - Schaecher, K.
AU - Rocchini, A.
AU - Dinkins, J.
AU - Matzelle, D. D.
AU - Banik, N. L.
N1 - Funding Information:
This work was supported in part by grants from NIH (NS-3166, NS38146, and NS41088). J. Dinkins was supported by the MUSC Summer Health Science Student Research Program.
PY - 2002
Y1 - 2002
N2 - Calpain activity and expression at the protein level were examined in inflammatory cells, activated microglia, and astrocytes prior to or at onset of symptomatic experimental allergic encephalomyelitis (EAE), an animal model for the human demyelinating disease multiple sclerosis (MS). EAE was induced in Lewis rats by injection of guinea pig spinal cord homogenate and myelin basic protein (MBP) emulsified with Complete Freund's Adjuvant (CFA). Calpain translational expression, determined by Western blot and immunocytochemistry, was correlated with calpain activity, infiltration of inflammatory cells, and myelin loss at 2-11 days following challenge with antigen. Controls (CFA only) did not show any changes over time in these parameters and very few changes (CD11+ microglia/mononuclear phagocytes) were seen in either group from days 2 to 8 post-induction. In contrast, from days 9 to 11, the animals that developed the disease (at least grade 1) demonstrated extensive cellular infiltration (CD4+, CD25+, and CD11+) as well as increased calpain expression (content) and activity. This study demonstrates that cell infiltration and increased calpain activity do not begin in the CNS until the onset of clinical signs.
AB - Calpain activity and expression at the protein level were examined in inflammatory cells, activated microglia, and astrocytes prior to or at onset of symptomatic experimental allergic encephalomyelitis (EAE), an animal model for the human demyelinating disease multiple sclerosis (MS). EAE was induced in Lewis rats by injection of guinea pig spinal cord homogenate and myelin basic protein (MBP) emulsified with Complete Freund's Adjuvant (CFA). Calpain translational expression, determined by Western blot and immunocytochemistry, was correlated with calpain activity, infiltration of inflammatory cells, and myelin loss at 2-11 days following challenge with antigen. Controls (CFA only) did not show any changes over time in these parameters and very few changes (CD11+ microglia/mononuclear phagocytes) were seen in either group from days 2 to 8 post-induction. In contrast, from days 9 to 11, the animals that developed the disease (at least grade 1) demonstrated extensive cellular infiltration (CD4+, CD25+, and CD11+) as well as increased calpain expression (content) and activity. This study demonstrates that cell infiltration and increased calpain activity do not begin in the CNS until the onset of clinical signs.
KW - Cell activation
KW - Demyelination
KW - Microglia
KW - T lymphocytes
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U2 - 10.1016/S0165-5728(02)00142-X
DO - 10.1016/S0165-5728(02)00142-X
M3 - Article
C2 - 12161014
AN - SCOPUS:0036343839
SN - 0165-5728
VL - 129
SP - 1
EP - 9
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -