Caloric restriction prevents the development of airway hyperresponsiveness in mice on a high fat diet

Haris Younas, Marcela Vieira, Chenjuan Gu, Rachel Lee, Mi-Kyung Shin, Slava Berger, Jeff Loube, Andrew Nelson, Shannon Bevans-Fonti, Qiong Zhong, Franco D'Alessio, Meredith McCormack, Nadia Hansel, Wayne A Mitzner, Vsevolod Polotsky

Research output: Contribution to journalArticle

Abstract

We have previously shown that high fat diet (HFD) for 2 weeks increases airway hyperresponsiveness (AHR) to methacholine challenge in C57BL/6J mice in association with an increase in IL-1β levels in lung tissue. We hypothesize that obesity increases AHR via the IL-1β mechanism, which can be prevented by caloric restriction and IL-1β blockade. In this study, we fed C57BL/6J mice for 8 weeks with several hypercaloric diets, including HFD, HFD supplemented with fructose, high trans-fat diet (HTFD) supplemented with fructose, either ad libitum or restricting their food intake to match body weight to the mice on a chow diet (CD). We also assessed the effect of the IL-1β receptor blocker anakinra. All mice showed the same total respiratory resistance at baseline. All obese mice showed higher AHR at 30 mg/ml of methacholine compared to CD and food restricted groups, regardless of the diet. Obese mice showed significant increases in lung IL-1 β mRNA expression, but not the protein, compared to CD and food restricted mice. Anakinra abolished an increase in AHR. We conclude that obesity leads to the airway hyperresponsiveness preventable by caloric restriction and IL-1β blockade.

Original languageEnglish (US)
Article number279
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Caloric Restriction
High Fat Diet
Interleukin-1
Diet
Interleukin 1 Receptor Antagonist Protein
Obese Mice
Methacholine Chloride
Fructose
Inbred C57BL Mouse
Obesity
Food
Lung
Interleukin-1 Receptors
Eating
Body Weight
Messenger RNA
Proteins

ASJC Scopus subject areas

  • General

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Caloric restriction prevents the development of airway hyperresponsiveness in mice on a high fat diet. / Younas, Haris; Vieira, Marcela; Gu, Chenjuan; Lee, Rachel; Shin, Mi-Kyung; Berger, Slava; Loube, Jeff; Nelson, Andrew; Bevans-Fonti, Shannon; Zhong, Qiong; D'Alessio, Franco; McCormack, Meredith; Hansel, Nadia; Mitzner, Wayne A; Polotsky, Vsevolod.

In: Scientific Reports, Vol. 9, No. 1, 279, 01.12.2019.

Research output: Contribution to journalArticle

Younas, Haris ; Vieira, Marcela ; Gu, Chenjuan ; Lee, Rachel ; Shin, Mi-Kyung ; Berger, Slava ; Loube, Jeff ; Nelson, Andrew ; Bevans-Fonti, Shannon ; Zhong, Qiong ; D'Alessio, Franco ; McCormack, Meredith ; Hansel, Nadia ; Mitzner, Wayne A ; Polotsky, Vsevolod. / Caloric restriction prevents the development of airway hyperresponsiveness in mice on a high fat diet. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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