Abstract
Calcineurin is responsible for mediating a wide variety of cellular processes in response to dynamic calcium (Ca(2+)) signals, yet the precise mechanisms involved in the spatiotemporal control of calcineurin signaling are poorly understood. Here, we use genetically encoded fluorescent biosensors to directly probe the role of cytosolic Ca(2+) oscillations in modulating calcineurin activity dynamics in insulin-secreting MIN6 β-cells. We show that Ca(2+) oscillations induce distinct temporal patterns of calcineurin activity in the cytosol and plasma membrane vs at the ER and mitochondria in these cells. Furthermore, we found that these differential calcineurin activity patterns are determined by variations in the subcellular distribution of calmodulin (CaM), indicating that CaM plays an active role in shaping both the spatial and temporal aspects of calcineurin signaling. Together, our findings provide new insights into the mechanisms by which oscillatory signals are decoded to generate specific functional outputs within different cellular compartments.
Original language | English (US) |
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Pages (from-to) | e03765 |
Journal | eLife |
Volume | 3 |
DOIs | |
State | Published - 2014 |
Keywords
- calcium
- FRET
- live-cell imaging
- oscillation
ASJC Scopus subject areas
- General Neuroscience
- General Medicine
- General Immunology and Microbiology
- General Biochemistry, Genetics and Molecular Biology