Calcium/calmodulin-activated phosphodiesterase expressed in olfactory receptor neurons

F. F. Borisy, G. V. Ronnett, A. M. Cunningham, D. Juilfs, J. Beavo, S. H. Snyder

Research output: Contribution to journalArticlepeer-review

Abstract

We show that calmodulin-dependent phosphodiesterase (CAM-PDE) is selectively expressed in mature olfactory receptor neurons within the olfactory mucosa. Immunocytochemical staining reveals neuronal immunoreactivity that is most pronounced within cilia, dendritic knobs, and axon bundles. Neither sustentacular cells nor basal cells display immunoreactivity. The extent of loss of neuronal immunoreactivity following bulbectomy resembles loss of the neuronal population. High-affinity CAM-PDE activity in olfactory cilia is fivefold greater than in brain, when assayed at low micromolar cAMP. This activity is depleted in turbinates following bulbectomy. Olfactory mucosal PDE activity is composed of a minimum of two major forms. In the absence of Ca2+, rolipram-sensitive PDE comprises 65% of total activity. Following stimulation by Ca2+, CAM-PDE activity is elevated sixfold to become the predominant form, thereby increasing total activity 300%, with half-maximal effect at 1 μM Ca2+. We propose that Ca2+ stimulation of CAM-PDE may be necessary for termination of olfactory signals.

Original languageEnglish (US)
Pages (from-to)915-923
Number of pages9
JournalJournal of Neuroscience
Volume12
Issue number3
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Neuroscience(all)

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