Calcium-sensitive particulate guanylyl cyclase as a modulator of cAMP in olfactory receptor neurons

Cheil Moon, Parham Jaberi, Annie Otto-Bruc, Wolfgang Baehr, Krzysztof Palczewski, Gabriele V. Ronnett

Research output: Contribution to journalArticlepeer-review

Abstract

The second messengers cAMP and inositol-1,4,5-triphosphate have been implicated in olfaction in various species. The odorant-induced cGMP response was investigated using cilia preparations and olfactory primary cultures. Odorants cause a delayed and sustained elevation of cGMR A component of this cGMP response is attributable to the activation of one of two kinetically distinct cilial receptor guanylyl cyclases by calcium and a guanylyl cyclase- activating protein (GCAP). cGMP thus formed serves to augment the cAMP signal in a cGMP-dependent protein kinase (PKG) manner by direct activation of adenylate cyclase. cAMP, in turn, activates cAMP-dependent protein kinase (PKA) to negatively regulate guanylyl cyclase, limiting the cGMP signal. These data demonstrate the existence of a regulatory loop in which cGMP can augment a cAMP signal, and in turn cAMP negatively regulates cGMP production via PKA. Thus, a small, localized, odorant-induced cAMP response may be amplified to modulate downstream transduction enzymes or transcriptional events.

Original languageEnglish (US)
Pages (from-to)3195-3205
Number of pages11
JournalJournal of Neuroscience
Volume18
Issue number9
StatePublished - May 1 1998

Keywords

  • Cilia
  • Guanylyl cyclase
  • Olfaction
  • Olfactory receptor neuron
  • Protein kinase
  • Signal transduction

ASJC Scopus subject areas

  • Neuroscience(all)

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