TY - JOUR
T1 - Calcium-sensitive particulate guanylyl cyclase as a modulator of cAMP in olfactory receptor neurons
AU - Moon, Cheil
AU - Jaberi, Parham
AU - Otto-Bruc, Annie
AU - Baehr, Wolfgang
AU - Palczewski, Krzysztof
AU - Ronnett, Gabriele V.
PY - 1998/5/1
Y1 - 1998/5/1
N2 - The second messengers cAMP and inositol-1,4,5-triphosphate have been implicated in olfaction in various species. The odorant-induced cGMP response was investigated using cilia preparations and olfactory primary cultures. Odorants cause a delayed and sustained elevation of cGMR A component of this cGMP response is attributable to the activation of one of two kinetically distinct cilial receptor guanylyl cyclases by calcium and a guanylyl cyclase- activating protein (GCAP). cGMP thus formed serves to augment the cAMP signal in a cGMP-dependent protein kinase (PKG) manner by direct activation of adenylate cyclase. cAMP, in turn, activates cAMP-dependent protein kinase (PKA) to negatively regulate guanylyl cyclase, limiting the cGMP signal. These data demonstrate the existence of a regulatory loop in which cGMP can augment a cAMP signal, and in turn cAMP negatively regulates cGMP production via PKA. Thus, a small, localized, odorant-induced cAMP response may be amplified to modulate downstream transduction enzymes or transcriptional events.
AB - The second messengers cAMP and inositol-1,4,5-triphosphate have been implicated in olfaction in various species. The odorant-induced cGMP response was investigated using cilia preparations and olfactory primary cultures. Odorants cause a delayed and sustained elevation of cGMR A component of this cGMP response is attributable to the activation of one of two kinetically distinct cilial receptor guanylyl cyclases by calcium and a guanylyl cyclase- activating protein (GCAP). cGMP thus formed serves to augment the cAMP signal in a cGMP-dependent protein kinase (PKG) manner by direct activation of adenylate cyclase. cAMP, in turn, activates cAMP-dependent protein kinase (PKA) to negatively regulate guanylyl cyclase, limiting the cGMP signal. These data demonstrate the existence of a regulatory loop in which cGMP can augment a cAMP signal, and in turn cAMP negatively regulates cGMP production via PKA. Thus, a small, localized, odorant-induced cAMP response may be amplified to modulate downstream transduction enzymes or transcriptional events.
KW - Cilia
KW - Guanylyl cyclase
KW - Olfaction
KW - Olfactory receptor neuron
KW - Protein kinase
KW - Signal transduction
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M3 - Article
C2 - 9547228
AN - SCOPUS:0032079821
SN - 0270-6474
VL - 18
SP - 3195
EP - 3205
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 9
ER -