Calcium sensing receptor: Molecular cloning in rat and localization to nerve terminals

M. Ruat, M. E. Molliver, Adele M Snowman, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

We have molecularly cloned a calcium sensing receptor (CaSR) from a rat striatal cDNA library. Rat CaSR displays 92% overall homology to its bovine counterpart with seven putative transmembrane domains characteristic of the superfamily of guanine nucleotide-binding proteins and significant homology with the metabotropic glutamate receptors. Northern blot analysis reveals two transcripts in thyroid, kidney, lung, ileum, and pituitary. In brain highest regional expression of the RNA occurs in the hypothalamus and the corpus striatum. Immunohistochemistry reveals discrete punctate localizations throughout the brain that appear to be associated with nerve terminals. No staining is evident in cell bodies of neurons or glia. Cerebral arteries display an intense network of CaSR immunoreactive fibers associated with vessel innervation. CaSR on nerve terminal membranes may regulate neurotransmitter disposition in response to Ca2+ levels in the synaptic space.

Original languageEnglish (US)
Pages (from-to)3161-3165
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number8
StatePublished - 1995

Fingerprint

Calcium-Sensing Receptors
Molecular Cloning
Corpus Striatum
Metabotropic Glutamate Receptors
Cerebral Arteries
Guanine Nucleotides
Brain
Gene Library
Ileum
Neuroglia
Northern Blotting
Hypothalamus
Neurotransmitter Agents
Carrier Proteins
Thyroid Gland
Immunohistochemistry
RNA
Staining and Labeling
Kidney
Neurons

Keywords

  • cerebral blood vessel
  • kidney
  • parathyroid
  • pituitary
  • thyroid hormone

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

@article{03c1403910a5409886018071f73314b5,
title = "Calcium sensing receptor: Molecular cloning in rat and localization to nerve terminals",
abstract = "We have molecularly cloned a calcium sensing receptor (CaSR) from a rat striatal cDNA library. Rat CaSR displays 92{\%} overall homology to its bovine counterpart with seven putative transmembrane domains characteristic of the superfamily of guanine nucleotide-binding proteins and significant homology with the metabotropic glutamate receptors. Northern blot analysis reveals two transcripts in thyroid, kidney, lung, ileum, and pituitary. In brain highest regional expression of the RNA occurs in the hypothalamus and the corpus striatum. Immunohistochemistry reveals discrete punctate localizations throughout the brain that appear to be associated with nerve terminals. No staining is evident in cell bodies of neurons or glia. Cerebral arteries display an intense network of CaSR immunoreactive fibers associated with vessel innervation. CaSR on nerve terminal membranes may regulate neurotransmitter disposition in response to Ca2+ levels in the synaptic space.",
keywords = "cerebral blood vessel, kidney, parathyroid, pituitary, thyroid hormone",
author = "M. Ruat and Molliver, {M. E.} and Snowman, {Adele M} and Snyder, {Solomon H}",
year = "1995",
language = "English (US)",
volume = "92",
pages = "3161--3165",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "8",

}

TY - JOUR

T1 - Calcium sensing receptor

T2 - Molecular cloning in rat and localization to nerve terminals

AU - Ruat, M.

AU - Molliver, M. E.

AU - Snowman, Adele M

AU - Snyder, Solomon H

PY - 1995

Y1 - 1995

N2 - We have molecularly cloned a calcium sensing receptor (CaSR) from a rat striatal cDNA library. Rat CaSR displays 92% overall homology to its bovine counterpart with seven putative transmembrane domains characteristic of the superfamily of guanine nucleotide-binding proteins and significant homology with the metabotropic glutamate receptors. Northern blot analysis reveals two transcripts in thyroid, kidney, lung, ileum, and pituitary. In brain highest regional expression of the RNA occurs in the hypothalamus and the corpus striatum. Immunohistochemistry reveals discrete punctate localizations throughout the brain that appear to be associated with nerve terminals. No staining is evident in cell bodies of neurons or glia. Cerebral arteries display an intense network of CaSR immunoreactive fibers associated with vessel innervation. CaSR on nerve terminal membranes may regulate neurotransmitter disposition in response to Ca2+ levels in the synaptic space.

AB - We have molecularly cloned a calcium sensing receptor (CaSR) from a rat striatal cDNA library. Rat CaSR displays 92% overall homology to its bovine counterpart with seven putative transmembrane domains characteristic of the superfamily of guanine nucleotide-binding proteins and significant homology with the metabotropic glutamate receptors. Northern blot analysis reveals two transcripts in thyroid, kidney, lung, ileum, and pituitary. In brain highest regional expression of the RNA occurs in the hypothalamus and the corpus striatum. Immunohistochemistry reveals discrete punctate localizations throughout the brain that appear to be associated with nerve terminals. No staining is evident in cell bodies of neurons or glia. Cerebral arteries display an intense network of CaSR immunoreactive fibers associated with vessel innervation. CaSR on nerve terminal membranes may regulate neurotransmitter disposition in response to Ca2+ levels in the synaptic space.

KW - cerebral blood vessel

KW - kidney

KW - parathyroid

KW - pituitary

KW - thyroid hormone

UR - http://www.scopus.com/inward/record.url?scp=0028955536&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028955536&partnerID=8YFLogxK

M3 - Article

C2 - 7724534

AN - SCOPUS:0028955536

VL - 92

SP - 3161

EP - 3165

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 8

ER -