Calcium sensing receptor: Molecular cloning in rat and localization to nerve terminals

Martial Ruat, Mark E. Molliver, Adele M. Snowman, Solomon H. Snyder

Research output: Contribution to journalArticle

Abstract

We have molecularly cloned a calcium sensing receptor (CaSR) from a rat striatal cDNA library. Rat CaSR displays 92% overall homology to its bovine counterpart with seven putative transmembrane domains characteristic of the superfamily of guanine nucleotide-binding proteins and significant homology with the metabotropic glutamate receptors. Northern blot analysis reveals two transcripts in thyroid, kidney, lung, ileum, and pituitary. In brain highest regional expression of the RNA occurs in the hypothalamus and the corpus striatum. Immunohistochemistry reveals discrete punctate localizations throughout the brain that appear to be associated with nerve terminals. No staining is evident in cell bodies of neurons or glia. Cerebral arteries display an intense network of CaSR immunoreactive fibers associated with vessel innervation. CaSR on nerve terminal membranes may regulate neurotransmitter disposition in response to Ca2+ levels in the synaptic space.

Original languageEnglish (US)
Pages (from-to)3161-3165
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number8
DOIs
StatePublished - Apr 11 1995

Keywords

  • cerebral blood vessel
  • kidney
  • parathyroid
  • pituitary
  • thyroid hormone

ASJC Scopus subject areas

  • General

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