@article{bf4c0a718c9a470da402d797c5144d06,
title = "Calcium dynamics in astrocyte processes during neurovascular coupling",
abstract = "Enhanced neuronal activity in the brain triggers a local increase in blood flow, termed functional hyperemia, via several mechanisms, including calcium (Ca 2+) signaling in astrocytes. However, recent in vivo studies have questioned the role of astrocytes in functional hyperemia because of the slow and sparse dynamics of their somatic Ca 2+ signals and the absence of glutamate metabotropic receptor 5 in adults. Here, we reexamined their role in neurovascular coupling by selectively expressing a genetically encoded Ca 2+ sensor in astrocytes of the olfactory bulb. We show that in anesthetized mice, the physiological activation of olfactory sensory neuron (OSN) terminals reliably triggers Ca 2+ increases in astrocyte processes but not in somata. These Ca 2+ increases systematically precede the onset of functional hyperemia by 1-2 s, reestablishing astrocytes as potential regulators of neurovascular coupling.",
author = "Yo Otsu and Kiri Couchman and Lyons, {Declan G.} and Mayeul Collot and Amit Agarwal and Mallet, {Jean Maurice} and Pfrieger, {Frank W.} and Bergles, {Dwight E.} and Serge Charpak",
note = "Funding Information: We would like to thank C. Pouzat for his critical comments. Support was provided by INSERM, the Agence Nationale de la Recherche (Project Angioneurins R11036KK), the Leducq Foundation, the Human Frontier Science Program Organization (RGP008912009-C), the Fondation pour la Recherche M{\'e}dicale (equipe FRM) and the US National Institutes of Health (MH084020 to D.E.B.). K.C. was supported by the Agence Nationale de la Recherche and the ERC Advanced Grant “Imaging-in-the-magnet.” D.G.L. was supported by a doctoral fellowship from {\'E}cole des Neurosciences de Paris (Paris School of Neuroscience) and a bursary from the Fondation pour la Recherche M{\'e}dicale (FDT20130928252). The team of S.C. is part of the {\'E}cole des Neurosciences de Paris Ile-de-France network. We thank W. Fr{\"o}stle (Ciba-Geigy) for the kind gift of CGP55845A (3-[1-(S)-(3,4-dichlorophenyl)ethyl]amino-2(S)hydroxypropyl-P-benzyl-phosphonic acid). Publisher Copyright: {\textcopyright} 2015 Nature America, Inc. All rights reserved.",
year = "2015",
month = feb,
day = "17",
doi = "10.1038/nn.3906",
language = "English (US)",
volume = "18",
pages = "210--218",
journal = "Nature Neuroscience",
issn = "1097-6256",
publisher = "Nature Publishing Group",
number = "2",
}