Calcium-dependent action potentials in mouse spinal cord neurons in cell culture

Eric J. Heyer, Robert L. Macdonald, Gregory K. Bergey, Phillip G. Nelson

Research output: Contribution to journalArticle

Abstract

Following blockade of membrane potassium conductance with tetraethylammonium ions or 3-aminopyridine, long-duration action potentials were recorded from mouse spinal cord neurons in primary dissociated cell culture. The action potentials were calcium-dependent since they: (1) were not blocked by the sodium-channel blocker tetrodotoxin, (2) could be recorded in sodium-free, calcium-containing medium (3) could not be evoked in sodium-containing, calcium-free medium, (4) were blocked by calcium channel blockers manganese and cobalt and (5) had overshot amplitudes that varied linearly with the log of the extracellular calcium concentration (slope of 27.5 mV/decade change in calcium concentration).

Original languageEnglish (US)
Pages (from-to)408-415
Number of pages8
JournalBrain research
Volume220
Issue number2
DOIs
StatePublished - Sep 14 1981
Externally publishedYes

Keywords

  • action potential
  • calcium
  • cell culture
  • spinal cord neurons

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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