Calcium and sperm components in the establishment of the membrane block to polyspermy: Studies of ICSI and activation with sperm factor

Genevieve B. Wortzman-Show, Manabu Kurokawa, Rafael A. Fissore, Janice Perry Evans

Research output: Contribution to journalArticle

Abstract

One important result of egg activation is the establishment of blocks to prevent polyspermic fertilization; these blocks are established on the zona pellucida and the egg plasma membrane. This study examines what the sperm brings to the egg to induce the establishment of the membrane block to polyspermy, building on past evidence that membrane block establishment does not occur in response to parthenogenetic stimuli that induce a single transient increase in cytosolic Ca2+ or intracytoplasmic sperm injection (ICSI). We test the hypotheses that (i) sperm-associated Ca2+ release activity triggers membrane block establishment; (ii) introduction of sperm contents via variations on ICSI protocols (resulting in improved Ca2+ transients, egg activation and embryo development over traditional ICSI protocols) triggers membrane block establishment and (iii) sperm adhesion [binding of an extracellular sperm ligand(s) to an egg receptor(s)] combined with sperm-associated Ca2+ release activity triggers membrane block establishment. Interestingly, none of these stimuli induced establishment of the membrane block to polyspermy in mouse eggs. However, the sperm-associated remodeling of the egg cortical cytoskeleton differs between conventionally fertilized and ICSI-fertilized eggs; taken with our previous data implicating actin microfilaments in membrane block establishment, this raises the possibility that cortical reorganization may be a contributing factor. In sum, fertilization-like Ca2+ transients, either alone or combined with sperm - egg binding, are not sufficient for membrane block establishment, but that an event(s) associated with gamete interaction plays a role in this membrane function change.

Original languageEnglish (US)
Pages (from-to)557-565
Number of pages9
JournalMolecular Human Reproduction
Volume13
Issue number8
DOIs
StatePublished - Aug 2007

Fingerprint

Intracytoplasmic Sperm Injections
Spermatozoa
Ovum
Calcium
Membranes
Fertilization
Sperm-Ovum Interactions
Zona Pellucida
Zygote
Cytoskeleton
Actin Cytoskeleton
Eggs
Embryonic Development
Cell Membrane
Ligands

Keywords

  • Calcium
  • Egg activation
  • Fertilization
  • ICSI
  • Polyspermy

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Genetics
  • Developmental Biology
  • Embryology
  • Cell Biology

Cite this

Calcium and sperm components in the establishment of the membrane block to polyspermy : Studies of ICSI and activation with sperm factor. / Wortzman-Show, Genevieve B.; Kurokawa, Manabu; Fissore, Rafael A.; Evans, Janice Perry.

In: Molecular Human Reproduction, Vol. 13, No. 8, 08.2007, p. 557-565.

Research output: Contribution to journalArticle

Wortzman-Show, Genevieve B. ; Kurokawa, Manabu ; Fissore, Rafael A. ; Evans, Janice Perry. / Calcium and sperm components in the establishment of the membrane block to polyspermy : Studies of ICSI and activation with sperm factor. In: Molecular Human Reproduction. 2007 ; Vol. 13, No. 8. pp. 557-565.
@article{ba00902beb464312881b72c0699610be,
title = "Calcium and sperm components in the establishment of the membrane block to polyspermy: Studies of ICSI and activation with sperm factor",
abstract = "One important result of egg activation is the establishment of blocks to prevent polyspermic fertilization; these blocks are established on the zona pellucida and the egg plasma membrane. This study examines what the sperm brings to the egg to induce the establishment of the membrane block to polyspermy, building on past evidence that membrane block establishment does not occur in response to parthenogenetic stimuli that induce a single transient increase in cytosolic Ca2+ or intracytoplasmic sperm injection (ICSI). We test the hypotheses that (i) sperm-associated Ca2+ release activity triggers membrane block establishment; (ii) introduction of sperm contents via variations on ICSI protocols (resulting in improved Ca2+ transients, egg activation and embryo development over traditional ICSI protocols) triggers membrane block establishment and (iii) sperm adhesion [binding of an extracellular sperm ligand(s) to an egg receptor(s)] combined with sperm-associated Ca2+ release activity triggers membrane block establishment. Interestingly, none of these stimuli induced establishment of the membrane block to polyspermy in mouse eggs. However, the sperm-associated remodeling of the egg cortical cytoskeleton differs between conventionally fertilized and ICSI-fertilized eggs; taken with our previous data implicating actin microfilaments in membrane block establishment, this raises the possibility that cortical reorganization may be a contributing factor. In sum, fertilization-like Ca2+ transients, either alone or combined with sperm - egg binding, are not sufficient for membrane block establishment, but that an event(s) associated with gamete interaction plays a role in this membrane function change.",
keywords = "Calcium, Egg activation, Fertilization, ICSI, Polyspermy",
author = "Wortzman-Show, {Genevieve B.} and Manabu Kurokawa and Fissore, {Rafael A.} and Evans, {Janice Perry}",
year = "2007",
month = "8",
doi = "10.1093/molehr/gam042",
language = "English (US)",
volume = "13",
pages = "557--565",
journal = "Molecular Human Reproduction",
issn = "1360-9947",
publisher = "Oxford University Press",
number = "8",

}

TY - JOUR

T1 - Calcium and sperm components in the establishment of the membrane block to polyspermy

T2 - Studies of ICSI and activation with sperm factor

AU - Wortzman-Show, Genevieve B.

AU - Kurokawa, Manabu

AU - Fissore, Rafael A.

AU - Evans, Janice Perry

PY - 2007/8

Y1 - 2007/8

N2 - One important result of egg activation is the establishment of blocks to prevent polyspermic fertilization; these blocks are established on the zona pellucida and the egg plasma membrane. This study examines what the sperm brings to the egg to induce the establishment of the membrane block to polyspermy, building on past evidence that membrane block establishment does not occur in response to parthenogenetic stimuli that induce a single transient increase in cytosolic Ca2+ or intracytoplasmic sperm injection (ICSI). We test the hypotheses that (i) sperm-associated Ca2+ release activity triggers membrane block establishment; (ii) introduction of sperm contents via variations on ICSI protocols (resulting in improved Ca2+ transients, egg activation and embryo development over traditional ICSI protocols) triggers membrane block establishment and (iii) sperm adhesion [binding of an extracellular sperm ligand(s) to an egg receptor(s)] combined with sperm-associated Ca2+ release activity triggers membrane block establishment. Interestingly, none of these stimuli induced establishment of the membrane block to polyspermy in mouse eggs. However, the sperm-associated remodeling of the egg cortical cytoskeleton differs between conventionally fertilized and ICSI-fertilized eggs; taken with our previous data implicating actin microfilaments in membrane block establishment, this raises the possibility that cortical reorganization may be a contributing factor. In sum, fertilization-like Ca2+ transients, either alone or combined with sperm - egg binding, are not sufficient for membrane block establishment, but that an event(s) associated with gamete interaction plays a role in this membrane function change.

AB - One important result of egg activation is the establishment of blocks to prevent polyspermic fertilization; these blocks are established on the zona pellucida and the egg plasma membrane. This study examines what the sperm brings to the egg to induce the establishment of the membrane block to polyspermy, building on past evidence that membrane block establishment does not occur in response to parthenogenetic stimuli that induce a single transient increase in cytosolic Ca2+ or intracytoplasmic sperm injection (ICSI). We test the hypotheses that (i) sperm-associated Ca2+ release activity triggers membrane block establishment; (ii) introduction of sperm contents via variations on ICSI protocols (resulting in improved Ca2+ transients, egg activation and embryo development over traditional ICSI protocols) triggers membrane block establishment and (iii) sperm adhesion [binding of an extracellular sperm ligand(s) to an egg receptor(s)] combined with sperm-associated Ca2+ release activity triggers membrane block establishment. Interestingly, none of these stimuli induced establishment of the membrane block to polyspermy in mouse eggs. However, the sperm-associated remodeling of the egg cortical cytoskeleton differs between conventionally fertilized and ICSI-fertilized eggs; taken with our previous data implicating actin microfilaments in membrane block establishment, this raises the possibility that cortical reorganization may be a contributing factor. In sum, fertilization-like Ca2+ transients, either alone or combined with sperm - egg binding, are not sufficient for membrane block establishment, but that an event(s) associated with gamete interaction plays a role in this membrane function change.

KW - Calcium

KW - Egg activation

KW - Fertilization

KW - ICSI

KW - Polyspermy

UR - http://www.scopus.com/inward/record.url?scp=34547789325&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547789325&partnerID=8YFLogxK

U2 - 10.1093/molehr/gam042

DO - 10.1093/molehr/gam042

M3 - Article

C2 - 17575288

AN - SCOPUS:34547789325

VL - 13

SP - 557

EP - 565

JO - Molecular Human Reproduction

JF - Molecular Human Reproduction

SN - 1360-9947

IS - 8

ER -