Cadmium and zinc flux in wild-type and cadmium-resistant CHO cells

Alice J. Corrigan, P. C. Huang

Research output: Contribution to journalArticlepeer-review

Abstract

Cellular flux of cadmium-109 and zinc-65 is characterized in cultured Chinese hamster ovary cells. The transport of cadmium is primarily unidirectional and, following uptake, cadmium is strongly retained. Zinc transport is bidirectional and intracellular zinc continuously leaches out into the medium. Nonradioactive cadmium or zinc enhances the efflux of65Zn from prelabeled cells. Transport of these metals into wild-type cells is not affected by azide, ouabain, cycloheximide, or actinomycin D. A cadmium-resistant mutant was isolated that exhibited altered sensitivities to certain inhibitors of macromolecular synthesis as well as quantitative differences in metal transport and accumulation. Although the mutant accumulates less cadmium than the wild-type cell, that which is retained is bound much more tightly. In addition, this lower rate of cadmium uptake is significantly decreased by either cycloheximide or actinomycin D. This suggests that the de novo synthesis of a protein or proteins is required for much of the net cadmium retention by the cadmium-resistant cells.

Original languageEnglish (US)
Pages (from-to)25-33
Number of pages9
JournalBiological Trace Element Research
Volume5
Issue number1
DOIs
StatePublished - Feb 1 1983

Keywords

  • CHO cells, Cd and Zn flux in
  • Cadmium, flux in CHO cells
  • antagonism, to Cd and Zn flux in CHO cells
  • metallothionein, and Cd and Zn flux in CHO cells
  • transport, of Cd and Zn flux in CHO cells
  • zinc, flux in CHO cells

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical
  • Inorganic Chemistry

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