Cadherin 99C regulates apical expansion and cell rearrangement during epithelial tube elongation

Se Yeon Chung, Deborah J. Andrew

Research output: Contribution to journalArticlepeer-review


Apical and basolateral determinants specify and maintain membrane domains in epithelia. Here, we identify new roles for two apical surface proteins - Cadherin 99C (Cad99C) and Stranded at Second (SAS) - in conferring apical character in Drosophila tubular epithelia. Cad99C, the Drosophila ortholog of human Usher protocadherin PCDH15, is expressed in several embryonic tubular epithelial structures. Through loss-of-function and overexpression studies, we show that Cad99C is required to regulate cell rearrangement during salivary tube elongation. We further show that overexpression of either Cad99C or SAS causes a dramatic increase in apical membrane at the expense of other membrane domains, and that both proteins can do this independently of each other and independently of mislocalization of the apical determinant Crumbs (Crb). Overexpression of Cad99C or SAS results in similar, but distinct effects, suggesting both shared and unique roles for these proteins in conferring apical identity.

Original languageEnglish (US)
Pages (from-to)1950-1960
Number of pages11
JournalDevelopment (Cambridge)
Issue number9
StatePublished - May 2014


  • Apicobasal polarity
  • Drosophila
  • Epithelial organ development
  • Salivary gland
  • Tubulogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Cadherin 99C regulates apical expansion and cell rearrangement during epithelial tube elongation'. Together they form a unique fingerprint.

Cite this