Abstract
We present a 13C direct detection CACA-TOCSY experiment for samples with alternate 13C-12C labeling. It provides inter-residue correlations between 13Cα resonances of residue i and adjacent Cαs at positions i - 1 and i + 1. Furthermore, longer mixing times yield correlations to Cα nuclei separated by more than one residue. The experiment also provides C α-to-sidechain correlations, some amino acid type identifications and estimates for ψ dihedral angles. The power of the experiment derives from the alternate 13C-12C labeling with [1,3-13C] glycerol or [2-13C] glycerol, which allows utilizing the small scalar 3JCC couplings that are masked by strong 1JCC couplings in uniformly 13C labeled samples.
Original language | English (US) |
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Pages (from-to) | 55-63 |
Number of pages | 9 |
Journal | Journal of Biomolecular NMR |
Volume | 47 |
Issue number | 1 |
DOIs | |
State | Published - May 2010 |
Keywords
- Alternate C labeling
- C direct detection
- Dihedral angle
- Nuclear magnetic resonance (NMR)
- Resonance assignment
- TOCSY
ASJC Scopus subject areas
- Biochemistry
- Spectroscopy