C1q/tumor necrosis factor-related protein 11 (CTRP11), a novel adipose stroma-derived regulator of adipogenesis

Zhikui Wei, Marcus M. Seldin, Niranjana Natarajan, David C. Djemal, Jonathan M. Peterson, Guang William Wong

Research output: Contribution to journalArticle

Abstract

C1q/TNF-related proteins (CTRPs) are a family of secreted regulators of glucose and lipid metabolism. Here, we describe CTRP11, a novel and phylogenetically conserved member of the C1q family. Our studies revealed that white and brown adipose are major tissues that express CTRP11, and its expression is acutely regulated by changes in metabolic state. Within white adipose tissue, CTRP11 is primarily expressed by stromal vascular cells. As a secreted multimeric protein, CTRP11 forms disulfide- linked oligomers. Although the conserved N-terminal Cys-28 and Cys-32 are dispensable for the assembly of higherorder oligomeric structures, they are unexpectedly involved in modulating protein secretion. When co-expressed, CTRP11 forms heteromeric complexes with closely related CTRP10, CTRP13, and CRF (CTRP14) via the C-terminal globular domains, combinatorial associations that potentially generate functionally distinct complexes. Functional studies revealed a role for CTRP11 in regulating adipogenesis. Ectopic expression of CTRP11 or exposure to recombinant protein inhibited differentiation of 3T3-L1 adipocytes. The expression of peroxisome proliferator-activated receptor- and CAAT/enhancer binding protein- , which drive the adipogenic gene program, was markedly suppressed by CTRP11. Impaired adipogenesis was caused by a CTRP11-mediated decrease in p42/44-MAPK signaling and inhibition of mitotic clonal expansion, a process essential for adipocyte differentiation in culture. These results implicate CTRP11 as a novel secreted regulator of adipogenesis and highlight the potential paracrine cross-talk between adipocytes and cells of the stromal vascular compartment in maintaining adipose tissue homeostasis.

Original languageEnglish (US)
Pages (from-to)10214-10229
Number of pages16
JournalJournal of Biological Chemistry
Volume288
Issue number15
DOIs
StatePublished - Apr 12 2013

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Adipogenesis
Tumor Necrosis Factor-alpha
Proteins
Adipocytes
Stromal Cells
Blood Vessels
Tissue homeostasis
Tissue
CCAAT-Enhancer-Binding Proteins
White Adipose Tissue
Peroxisome Proliferator-Activated Receptors
Brown Adipose Tissue
Mitogen-Activated Protein Kinase 1
Lipid Metabolism
Recombinant Proteins
Oligomers
Disulfides
Adipose Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

C1q/tumor necrosis factor-related protein 11 (CTRP11), a novel adipose stroma-derived regulator of adipogenesis. / Wei, Zhikui; Seldin, Marcus M.; Natarajan, Niranjana; Djemal, David C.; Peterson, Jonathan M.; Wong, Guang William.

In: Journal of Biological Chemistry, Vol. 288, No. 15, 12.04.2013, p. 10214-10229.

Research output: Contribution to journalArticle

Wei, Zhikui ; Seldin, Marcus M. ; Natarajan, Niranjana ; Djemal, David C. ; Peterson, Jonathan M. ; Wong, Guang William. / C1q/tumor necrosis factor-related protein 11 (CTRP11), a novel adipose stroma-derived regulator of adipogenesis. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 15. pp. 10214-10229.
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