C1q binding to dengue virus decreases levels of infection and inflammatory molecules transcription in THP-1 cells

Bruno Douradinha; Sean P. McBurney; Klecia M. Soares de Melo; Amanda P. Smith; Neel K. Krishna; Simon M. Barratt-Boyes; Jared D. Evans; Eduardo J.M. Nascimento; Ernesto T.A. Marques

doi:10.1016/j.virusres.2013.11.007

C1q binding to dengue virus decreases levels of infection and inflammatory molecules transcription in THP-1 cells

Bruno Douradinha, Sean P. McBurney, Klecia M. Soares de Melo, Amanda P. Smith, Neel K. Krishna, Simon M. Barratt-Boyes, Jared D. Evans, Eduardo J.M. Nascimento, Ernesto T.A. Marques

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Dengue virus infection elicits a spectrum of clinical presentations ranging from asymptomatic to severe disease. The mechanisms leading to severe dengue are not known, however it has been reported that the complement system is hyper-activated in severe dengue. Screening of complement proteins demonstrated that C1q, a pattern recognition molecule, can bind directly to dengue virus envelope protein and to whole dengue virus serotype 2. Incubation of dengue virus serotype 2 with C1q prior to infection of THP-1 cells led to decreased virus infectivity and modulation of mRNA expression of immunoregulatory molecules suggesting reduced inflammatory responses.

Original language	English (US)
Pages (from-to)	231-234
Number of pages	4
Journal	Virus Research
Volume	179
Issue number	1
DOIs	https://doi.org/10.1016/j.virusres.2013.11.007
State	Published - Jan 22 2014
Externally published	Yes

Keywords

C1q
Classical complement pathway
Dengue
Dengue fever
Dengue hemorrhagic fever
Viral persistence

ASJC Scopus subject areas

Virology
Infectious Diseases
Cancer Research

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10.1016/j.virusres.2013.11.007

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title = "C1q binding to dengue virus decreases levels of infection and inflammatory molecules transcription in THP-1 cells",

abstract = "Dengue virus infection elicits a spectrum of clinical presentations ranging from asymptomatic to severe disease. The mechanisms leading to severe dengue are not known, however it has been reported that the complement system is hyper-activated in severe dengue. Screening of complement proteins demonstrated that C1q, a pattern recognition molecule, can bind directly to dengue virus envelope protein and to whole dengue virus serotype 2. Incubation of dengue virus serotype 2 with C1q prior to infection of THP-1 cells led to decreased virus infectivity and modulation of mRNA expression of immunoregulatory molecules suggesting reduced inflammatory responses.",

keywords = "C1q, Classical complement pathway, Dengue, Dengue fever, Dengue hemorrhagic fever, Viral persistence",

author = "Bruno Douradinha and McBurney, {Sean P.} and {Soares de Melo}, {Klecia M.} and Smith, {Amanda P.} and Krishna, {Neel K.} and Barratt-Boyes, {Simon M.} and Evans, {Jared D.} and Nascimento, {Eduardo J.M.} and Marques, {Ernesto T.A.}",

note = "Funding Information: We thank the reviewers for their helpful comments and suggestions. B.D. is a Fondazione Ri.MED (Palermo, Italy) postdoctoral fellow. This work was partially supported by a National Institutes of Health grant R21 AI095750 to N.K.K. J.D.E. is supported by a grant from the Fine Foundation. ",

year = "2014",

month = jan,

day = "22",

doi = "10.1016/j.virusres.2013.11.007",

language = "English (US)",

volume = "179",

pages = "231--234",

journal = "Virus Research",

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T1 - C1q binding to dengue virus decreases levels of infection and inflammatory molecules transcription in THP-1 cells

AU - Douradinha, Bruno

AU - McBurney, Sean P.

AU - Soares de Melo, Klecia M.

AU - Smith, Amanda P.

AU - Krishna, Neel K.

AU - Barratt-Boyes, Simon M.

AU - Evans, Jared D.

AU - Nascimento, Eduardo J.M.

AU - Marques, Ernesto T.A.

N1 - Funding Information: We thank the reviewers for their helpful comments and suggestions. B.D. is a Fondazione Ri.MED (Palermo, Italy) postdoctoral fellow. This work was partially supported by a National Institutes of Health grant R21 AI095750 to N.K.K. J.D.E. is supported by a grant from the Fine Foundation.

PY - 2014/1/22

Y1 - 2014/1/22

N2 - Dengue virus infection elicits a spectrum of clinical presentations ranging from asymptomatic to severe disease. The mechanisms leading to severe dengue are not known, however it has been reported that the complement system is hyper-activated in severe dengue. Screening of complement proteins demonstrated that C1q, a pattern recognition molecule, can bind directly to dengue virus envelope protein and to whole dengue virus serotype 2. Incubation of dengue virus serotype 2 with C1q prior to infection of THP-1 cells led to decreased virus infectivity and modulation of mRNA expression of immunoregulatory molecules suggesting reduced inflammatory responses.

AB - Dengue virus infection elicits a spectrum of clinical presentations ranging from asymptomatic to severe disease. The mechanisms leading to severe dengue are not known, however it has been reported that the complement system is hyper-activated in severe dengue. Screening of complement proteins demonstrated that C1q, a pattern recognition molecule, can bind directly to dengue virus envelope protein and to whole dengue virus serotype 2. Incubation of dengue virus serotype 2 with C1q prior to infection of THP-1 cells led to decreased virus infectivity and modulation of mRNA expression of immunoregulatory molecules suggesting reduced inflammatory responses.

KW - C1q

KW - Classical complement pathway

KW - Dengue

KW - Dengue fever

KW - Dengue hemorrhagic fever

KW - Viral persistence

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C1q binding to dengue virus decreases levels of infection and inflammatory molecules transcription in THP-1 cells

Abstract

Keywords

ASJC Scopus subject areas

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