C-reactive protein relaxes human vessels in vitro

Leonid Sternik, Saquib Samee, Hartzel V. Schaff, Kenton J Zehr, Lilach O. Lerman, David R. Holmes, Joerg Herrmann, Amir Lerman

Research output: Contribution to journalArticle

Abstract

Objective - C-reactive protein (CRP) is a sensitive marker of inflammation and a prognostic marker in cardiovascular disease. Evidence suggests direct biological activities of CRP within the vascular wall. The study was designed to examine the vasoreactive effects of CRP. Methods and Results - Human internal mammary artery rings were obtained during cardiovascular bypass surgery and suspended in an organ bath chamber. The rings were precontracted with endothelin-1, and response to cumulative concentrations of CRP was obtained. Experiments were repeated after initial incubation with 20, 40, and 60 mmol/L KCl, the potassium channel blockers BaCl, tetraethylammonium chloride, and glibenclamide, and the NO synthase inhibitor N-monomethyl-L-arginine and also after removal of the endothelium. CRP caused dose-dependent relaxation of human internal mammary artery rings, which was not affected by preincubation with N-monomethyl-L-arginine or removal of the endothelium. Maximum relaxation response to CRP (79.5±10%) was attenuated by KCl (2.5±11.5%, P

Original languageEnglish (US)
Pages (from-to)1865-1868
Number of pages4
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume22
Issue number11
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

C-Reactive Protein
Mammary Arteries
Endothelium
Arginine
Potassium Channel Blockers
Tetraethylammonium
Glyburide
Endothelin-1
Baths
Nitric Oxide Synthase
Blood Vessels
In Vitro Techniques
Cardiovascular Diseases
Inflammation

Keywords

  • Atherosclerosis
  • C-reactive protein
  • Inflammation
  • Potassium channels
  • Vasorelaxation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Sternik, L., Samee, S., Schaff, H. V., Zehr, K. J., Lerman, L. O., Holmes, D. R., ... Lerman, A. (2002). C-reactive protein relaxes human vessels in vitro. Arteriosclerosis, Thrombosis, and Vascular Biology, 22(11), 1865-1868. https://doi.org/10.1161/01.ATV.0000033821.96354.90

C-reactive protein relaxes human vessels in vitro. / Sternik, Leonid; Samee, Saquib; Schaff, Hartzel V.; Zehr, Kenton J; Lerman, Lilach O.; Holmes, David R.; Herrmann, Joerg; Lerman, Amir.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 22, No. 11, 01.11.2002, p. 1865-1868.

Research output: Contribution to journalArticle

Sternik, L, Samee, S, Schaff, HV, Zehr, KJ, Lerman, LO, Holmes, DR, Herrmann, J & Lerman, A 2002, 'C-reactive protein relaxes human vessels in vitro', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 22, no. 11, pp. 1865-1868. https://doi.org/10.1161/01.ATV.0000033821.96354.90
Sternik L, Samee S, Schaff HV, Zehr KJ, Lerman LO, Holmes DR et al. C-reactive protein relaxes human vessels in vitro. Arteriosclerosis, Thrombosis, and Vascular Biology. 2002 Nov 1;22(11):1865-1868. https://doi.org/10.1161/01.ATV.0000033821.96354.90
Sternik, Leonid ; Samee, Saquib ; Schaff, Hartzel V. ; Zehr, Kenton J ; Lerman, Lilach O. ; Holmes, David R. ; Herrmann, Joerg ; Lerman, Amir. / C-reactive protein relaxes human vessels in vitro. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2002 ; Vol. 22, No. 11. pp. 1865-1868.
@article{1ca009ff7dab435587de021c2547790a,
title = "C-reactive protein relaxes human vessels in vitro",
abstract = "Objective - C-reactive protein (CRP) is a sensitive marker of inflammation and a prognostic marker in cardiovascular disease. Evidence suggests direct biological activities of CRP within the vascular wall. The study was designed to examine the vasoreactive effects of CRP. Methods and Results - Human internal mammary artery rings were obtained during cardiovascular bypass surgery and suspended in an organ bath chamber. The rings were precontracted with endothelin-1, and response to cumulative concentrations of CRP was obtained. Experiments were repeated after initial incubation with 20, 40, and 60 mmol/L KCl, the potassium channel blockers BaCl, tetraethylammonium chloride, and glibenclamide, and the NO synthase inhibitor N-monomethyl-L-arginine and also after removal of the endothelium. CRP caused dose-dependent relaxation of human internal mammary artery rings, which was not affected by preincubation with N-monomethyl-L-arginine or removal of the endothelium. Maximum relaxation response to CRP (79.5±10{\%}) was attenuated by KCl (2.5±11.5{\%}, P",
keywords = "Atherosclerosis, C-reactive protein, Inflammation, Potassium channels, Vasorelaxation",
author = "Leonid Sternik and Saquib Samee and Schaff, {Hartzel V.} and Zehr, {Kenton J} and Lerman, {Lilach O.} and Holmes, {David R.} and Joerg Herrmann and Amir Lerman",
year = "2002",
month = "11",
day = "1",
doi = "10.1161/01.ATV.0000033821.96354.90",
language = "English (US)",
volume = "22",
pages = "1865--1868",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams and Wilkins",
number = "11",

}

TY - JOUR

T1 - C-reactive protein relaxes human vessels in vitro

AU - Sternik, Leonid

AU - Samee, Saquib

AU - Schaff, Hartzel V.

AU - Zehr, Kenton J

AU - Lerman, Lilach O.

AU - Holmes, David R.

AU - Herrmann, Joerg

AU - Lerman, Amir

PY - 2002/11/1

Y1 - 2002/11/1

N2 - Objective - C-reactive protein (CRP) is a sensitive marker of inflammation and a prognostic marker in cardiovascular disease. Evidence suggests direct biological activities of CRP within the vascular wall. The study was designed to examine the vasoreactive effects of CRP. Methods and Results - Human internal mammary artery rings were obtained during cardiovascular bypass surgery and suspended in an organ bath chamber. The rings were precontracted with endothelin-1, and response to cumulative concentrations of CRP was obtained. Experiments were repeated after initial incubation with 20, 40, and 60 mmol/L KCl, the potassium channel blockers BaCl, tetraethylammonium chloride, and glibenclamide, and the NO synthase inhibitor N-monomethyl-L-arginine and also after removal of the endothelium. CRP caused dose-dependent relaxation of human internal mammary artery rings, which was not affected by preincubation with N-monomethyl-L-arginine or removal of the endothelium. Maximum relaxation response to CRP (79.5±10%) was attenuated by KCl (2.5±11.5%, P

AB - Objective - C-reactive protein (CRP) is a sensitive marker of inflammation and a prognostic marker in cardiovascular disease. Evidence suggests direct biological activities of CRP within the vascular wall. The study was designed to examine the vasoreactive effects of CRP. Methods and Results - Human internal mammary artery rings were obtained during cardiovascular bypass surgery and suspended in an organ bath chamber. The rings were precontracted with endothelin-1, and response to cumulative concentrations of CRP was obtained. Experiments were repeated after initial incubation with 20, 40, and 60 mmol/L KCl, the potassium channel blockers BaCl, tetraethylammonium chloride, and glibenclamide, and the NO synthase inhibitor N-monomethyl-L-arginine and also after removal of the endothelium. CRP caused dose-dependent relaxation of human internal mammary artery rings, which was not affected by preincubation with N-monomethyl-L-arginine or removal of the endothelium. Maximum relaxation response to CRP (79.5±10%) was attenuated by KCl (2.5±11.5%, P

KW - Atherosclerosis

KW - C-reactive protein

KW - Inflammation

KW - Potassium channels

KW - Vasorelaxation

UR - http://www.scopus.com/inward/record.url?scp=0036845090&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036845090&partnerID=8YFLogxK

U2 - 10.1161/01.ATV.0000033821.96354.90

DO - 10.1161/01.ATV.0000033821.96354.90

M3 - Article

VL - 22

SP - 1865

EP - 1868

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 11

ER -