C-reactive protein relaxes human vessels in vitro

Leonid Sternik, Saquib Samee, Hartzel V. Schaff, Kenton J Zehr, Lilach O. Lerman, David R. Holmes, Joerg Herrmann, Amir Lerman

Research output: Contribution to journalArticle

Abstract

Objective - C-reactive protein (CRP) is a sensitive marker of inflammation and a prognostic marker in cardiovascular disease. Evidence suggests direct biological activities of CRP within the vascular wall. The study was designed to examine the vasoreactive effects of CRP. Methods and Results - Human internal mammary artery rings were obtained during cardiovascular bypass surgery and suspended in an organ bath chamber. The rings were precontracted with endothelin-1, and response to cumulative concentrations of CRP was obtained. Experiments were repeated after initial incubation with 20, 40, and 60 mmol/L KCl, the potassium channel blockers BaCl, tetraethylammonium chloride, and glibenclamide, and the NO synthase inhibitor N-monomethyl-L-arginine and also after removal of the endothelium. CRP caused dose-dependent relaxation of human internal mammary artery rings, which was not affected by preincubation with N-monomethyl-L-arginine or removal of the endothelium. Maximum relaxation response to CRP (79.5±10%) was attenuated by KCl (2.5±11.5%, P

Original languageEnglish (US)
Pages (from-to)1865-1868
Number of pages4
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume22
Issue number11
DOIs
Publication statusPublished - Nov 1 2002
Externally publishedYes

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Keywords

  • Atherosclerosis
  • C-reactive protein
  • Inflammation
  • Potassium channels
  • Vasorelaxation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Sternik, L., Samee, S., Schaff, H. V., Zehr, K. J., Lerman, L. O., Holmes, D. R., ... Lerman, A. (2002). C-reactive protein relaxes human vessels in vitro. Arteriosclerosis, Thrombosis, and Vascular Biology, 22(11), 1865-1868. https://doi.org/10.1161/01.ATV.0000033821.96354.90