C-reactive protein and complement factor H in aged human eyes and eyes with age-related macular degeneration

Imran A. Bhutto, Takayuki Baba, Carol Merges, Vikash Juriasinghani, D. Scott McLeod, Gerard Anthony Lutty

Research output: Contribution to journalArticle

Abstract

Background: There is increasing evidence that inflammation and immune-mediated processes (complement activation) play an important role in age-related macular degeneration (AMD) pathogenesis. A genetic variation in the gene encoding complement factor H (CFH) and plasma levels of C-reactive protein (CRP), a systemic marker of subclinical inflammation, have consistently been shown to be associated with an increased risk for AMD. In the present study, we examined the immunolocalisation of CRP and CFH in aged control human donor eyes (n=10; mean age 79 years) and eyes with AMD (n=18; mean age 83 years). Methods: Alkaline phosphatase immunohistochemistry was performed using polyclonal antibodies against CRP and CFH on cryopreserved tissue sections from disc/macular blocks. Three independent masked observers scored the reaction product (0-8). Results: In aged control eyes, the retinal pigment epithelium/Bruch's membrane/choriocapillaris (RPE/BrM/CC) complex including intercapillary septa (ICS) had the most prominent immunostaining for CRP and CFH. CRP was significantly higher than controls in BrM/CC/ICS and choroidal stroma in early and wet AMD eyes (p

Original languageEnglish (US)
Pages (from-to)1323-1330
Number of pages8
JournalBritish Journal of Ophthalmology
Volume95
Issue number9
DOIs
StatePublished - Sep 2011

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Complement Factor H
Macular Degeneration
C-Reactive Protein
Bruch Membrane
Inflammation
Retinal Pigment Epithelium
Complement Activation
Alkaline Phosphatase
Immunohistochemistry
Antibodies
Genes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

C-reactive protein and complement factor H in aged human eyes and eyes with age-related macular degeneration. / Bhutto, Imran A.; Baba, Takayuki; Merges, Carol; Juriasinghani, Vikash; McLeod, D. Scott; Lutty, Gerard Anthony.

In: British Journal of Ophthalmology, Vol. 95, No. 9, 09.2011, p. 1323-1330.

Research output: Contribution to journalArticle

Bhutto, Imran A. ; Baba, Takayuki ; Merges, Carol ; Juriasinghani, Vikash ; McLeod, D. Scott ; Lutty, Gerard Anthony. / C-reactive protein and complement factor H in aged human eyes and eyes with age-related macular degeneration. In: British Journal of Ophthalmology. 2011 ; Vol. 95, No. 9. pp. 1323-1330.
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