C-Myc modulates microRNA processing via the transcriptional regulation of Drosha

Xingwu Wang, Xiaocheng Zhao, Ping Gao, Mian Wu

Research output: Contribution to journalArticlepeer-review

Abstract

The c-Myc oncogenic transcription factor is known to regulate microRNA (miRNA) expression at the transcriptional level. However, little is known about the function of c-Myc in miRNA processing. Here, we report that Drosha, one of the most important components of the miRNA processing machinery, is a c-Myc target gene. c-Myc transactivates drosha mRNA expression, thus upregulating the Drosha protein level. A chromatin immunoprecipitation (ChIP) experiment revealed that c-Myc binds directly to the E-box of the drosha promoter. Both in vitro andin vivo microRNA processing assays demonstrated that c-Myc promotes miRNA processing by upregulating the Drosha expression level. Overall, our study reveals a previously unrecognised function of c-Myc in miRNA processing and provides valuable insight into a new aspect of how c-Myc regulates microRNA expression.

Original languageEnglish (US)
Article number1942
JournalScientific reports
Volume3
DOIs
StatePublished - Jun 5 2013

ASJC Scopus subject areas

  • General

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