c-Fos protein in photoreceptor cell death after photic injury in rats

H. K. Poon, M. O.M. Tso, T. T. Lam

Research output: Contribution to journalArticlepeer-review


PURPOSE. To examine the involvement of c-Fos protein in light-induced photoreceptor cell death in rats. METHODS. Thirty-two Lewis albino rats were exposed to green fluorescent light (480-520 nm) of 300 to 320 foot-candles (3228-3443.2 lux) for 3 hours, allowed to recover in the dark, and euthanatized at O, 1, 3, 6, 12, 24, or 96 hours after light exposure. c-Fos was detected immunohistochemically and nicked DNA by in situ TdT-dUTP terminal nick-end labeling (TUNEL). Double labeling of c-Fos and DNA nicks was also performed. RESULTS. There was a time-dependent change in the number of c-Fos-positive photoreceptor nuclei after light injury, which parceled the change in the number of TUNEL-positive nuclei. The temporal and spatial appearance of these nuclei also matched the appearance of pyknotic nuclei of the outer nuclear layer. Double-labeling study revealed that some c-Fos-positive nuclei were also TUNEL-positive nuclei. CONCLUSIONS. There was an acute accumulation of c-Fos protein in photoreceptor associated with cell death. This study further supports other studies showing that c-Fos is linked to apoptotic photoreceptor cell death.

Original languageEnglish (US)
Pages (from-to)2755-2758
Number of pages4
JournalInvestigative Ophthalmology and Visual Science
Issue number9
StatePublished - Aug 29 2000
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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