TY - JOUR
T1 - Bypassing spermiogenesis for several generations does not have detrimental consequences on the fertility and neurobehavior of offspring
T2 - A study using the mouse
AU - Tamashiro, Kellie L.K.
AU - Kimura, Yasuyuki
AU - Blanchard, Robert J.
AU - Caroline Blanchard, D.
AU - Yanagimachi, Ryuzo
N1 - Funding Information:
This study was supported by Grant HD-34362 from the National Institute of Child Health and Human Development.
PY - 1999
Y1 - 1999
N2 - Purpose: This study was conducted to determine whether the omission of spermiogenesis and all prefertilization events for five generations in mice affects the fertility or behavior of offspring. Methods: Fifth-generation hybrM (C57BL/6 X DBA/2) mice were produced using round spermatid injection (ROSI). Control groups consisted of mice born after natural mating with and without sham operation. The growth, fertility, and behavior of offspring were compared. Behavior tests conducted assessed elementary reasoning (Krushinsky test), emotionality (Mouse Defense Test Battery), and spatial learning and memory (Morris water maze). Results: There were no significant differences in the growth and fertility of fifth-generation ROSI mice compared to natural fertilization mice. We also found no evidence of significant learning or behavioral deficits of the fifth-generation ROSI mice. Conclusions: in this study, we found no evidence that bypassing the natural biological processes involved in spermiogenesis produces adverse effects on the growth, fertility, or behavior of mouse offspring.
AB - Purpose: This study was conducted to determine whether the omission of spermiogenesis and all prefertilization events for five generations in mice affects the fertility or behavior of offspring. Methods: Fifth-generation hybrM (C57BL/6 X DBA/2) mice were produced using round spermatid injection (ROSI). Control groups consisted of mice born after natural mating with and without sham operation. The growth, fertility, and behavior of offspring were compared. Behavior tests conducted assessed elementary reasoning (Krushinsky test), emotionality (Mouse Defense Test Battery), and spatial learning and memory (Morris water maze). Results: There were no significant differences in the growth and fertility of fifth-generation ROSI mice compared to natural fertilization mice. We also found no evidence of significant learning or behavioral deficits of the fifth-generation ROSI mice. Conclusions: in this study, we found no evidence that bypassing the natural biological processes involved in spermiogenesis produces adverse effects on the growth, fertility, or behavior of mouse offspring.
KW - Behavior
KW - Mouse
KW - Round spermatid injection
KW - Spermatid
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U2 - 10.1023/A:1020406016312
DO - 10.1023/A:1020406016312
M3 - Article
C2 - 10394528
AN - SCOPUS:0033005093
SN - 1058-0468
VL - 16
SP - 315
EP - 324
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
IS - 6
ER -