Bypassing spermiogenesis for several generations does not have detrimental consequences on the fertility and neurobehavior of offspring: A study using the mouse

Kellie L.K. Tamashiro, Yasuyuki Kimura, Robert J. Blanchard, D. Caroline Blanchard, Ryuzo Yanagimachi

Research output: Contribution to journalArticle

Abstract

Purpose: This study was conducted to determine whether the omission of spermiogenesis and all prefertilization events for five generations in mice affects the fertility or behavior of offspring. Methods: Fifth-generation hybrM (C57BL/6 X DBA/2) mice were produced using round spermatid injection (ROSI). Control groups consisted of mice born after natural mating with and without sham operation. The growth, fertility, and behavior of offspring were compared. Behavior tests conducted assessed elementary reasoning (Krushinsky test), emotionality (Mouse Defense Test Battery), and spatial learning and memory (Morris water maze). Results: There were no significant differences in the growth and fertility of fifth-generation ROSI mice compared to natural fertilization mice. We also found no evidence of significant learning or behavioral deficits of the fifth-generation ROSI mice. Conclusions: in this study, we found no evidence that bypassing the natural biological processes involved in spermiogenesis produces adverse effects on the growth, fertility, or behavior of mouse offspring.

Original languageEnglish (US)
Pages (from-to)315-324
Number of pages10
JournalJournal of Assisted Reproduction and Genetics
Volume16
Issue number6
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Keywords

  • Behavior
  • Mouse
  • Round spermatid injection
  • Spermatid

ASJC Scopus subject areas

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Genetics(clinical)

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