Broccoli (brassica oleracea var. italica) sprouts and extracts rich in glucosinolates and isothiocyanates affect cholesterol metabolism and genes involved in lipid homeostasis in hamsters

Laura N. Rodríguez-Cantú, Janet A. Gutiérrez-Uribe, Jennifer Arriola-Vucovich, Rocio I. Díaz-De La Garza, Jed W Fahey, Sergio O. Serna-Saldivar

Research output: Contribution to journalArticle

Abstract

This study investigated the effects of broccoli sprouts (BS) on sterol and lipid homeostasis in Syrian hamsters with dietary-induced hypercholesterolemia. Treatments included freeze-dried BS containing 2 or 20 μmol of glucoraphanine (BSX, BS10X), glucoraphanine-rich BS extract (GRE), sulforaphane-rich BS extract (SFE), and simvastatin. Each experimental diet was offered to eight animals (male and female) for 7 weeks. Hepatic cholesterol was reduced by BS10X and SFE treatments in all animals. This correlated with a down-regulation of gene expression of sterol regulatory element-binding proteins (SREBP-1 and -2) and fatty acid synthase (FAS) caused by GRE and SFE diets. BS10X caused changes in gene expression in a gender-specific manner; additionally, it increased coprostanol excretion in females. With the same concentration of glucoraphanin, consumption of broccoli sprouts (BS10X) had more marked effects on cholesterol homeostasis than GRE; this finding reinforces the importance of the matrix effects on the bioactivity of functional ingredients.

Original languageEnglish (US)
Pages (from-to)1095-1103
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number4
DOIs
StatePublished - Feb 23 2011

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Isothiocyanates
Brassica oleracea var. italica
Glucosinolates
sprouts (food)
cholesterol metabolism
Brassica
isothiocyanates
broccoli
glucosinolates
hamsters
Metabolism
Cricetinae
homeostasis
Homeostasis
Genes
Cholesterol
Nutrition
Lipids
Gene expression
extracts

Keywords

  • Apo B100
  • Broccoli sprouts
  • cholesterol
  • FAS
  • glucosinolate
  • HMGR
  • LDLR
  • SREBP
  • sulforaphane

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Broccoli (brassica oleracea var. italica) sprouts and extracts rich in glucosinolates and isothiocyanates affect cholesterol metabolism and genes involved in lipid homeostasis in hamsters. / Rodríguez-Cantú, Laura N.; Gutiérrez-Uribe, Janet A.; Arriola-Vucovich, Jennifer; Díaz-De La Garza, Rocio I.; Fahey, Jed W; Serna-Saldivar, Sergio O.

In: Journal of Agricultural and Food Chemistry, Vol. 59, No. 4, 23.02.2011, p. 1095-1103.

Research output: Contribution to journalArticle

Rodríguez-Cantú, Laura N. ; Gutiérrez-Uribe, Janet A. ; Arriola-Vucovich, Jennifer ; Díaz-De La Garza, Rocio I. ; Fahey, Jed W ; Serna-Saldivar, Sergio O. / Broccoli (brassica oleracea var. italica) sprouts and extracts rich in glucosinolates and isothiocyanates affect cholesterol metabolism and genes involved in lipid homeostasis in hamsters. In: Journal of Agricultural and Food Chemistry. 2011 ; Vol. 59, No. 4. pp. 1095-1103.
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