Abstract
The ability to accurately measure layered biological tissue optical properties (OPs) may improve understanding of spectroscopic device performance and facilitate early cancer detection. Towards these goals, we have performed theoretical and experimental evaluations of an approach for broadband measurement of absorption and reduced scattering coefficients at ultraviolet-visible wavelengths. Our technique is based on neural network (NN) inverse models trained with diffuse reflectance data from condensed Monte Carlo simulations. Experimental measurements were performed from 350 to 600 nm with a fiber-optic-based reflectance spectroscopy system. Two-layer phantoms incorporating OPs relevant to normal and dysplastic mucosal tissue and superficial layer thicknesses of 0.22 and 0.44 mm were used to assess prediction accuracy. Results showed mean OP estimation errors of 19% from the theoretical analysis and 27% from experiments. Two-step NN modeling and nonlinear spectral fitting approaches helped improve prediction accuracy. While limitations and challenges remain, the results of this study indicate that our technique can provide moderately accurate estimates of OPs in layered turbid media.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1226-1240 |
| Number of pages | 15 |
| Journal | Biomedical Optics Express |
| Volume | 3 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1 2012 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Biotechnology
Cite this
Broadband ultraviolet-visible optical property measurement in layered turbid media. / Wang, Quanzeng; Le, Du; Ramella-Roman, Jessica; Pfefer, Joshua.
In: Biomedical Optics Express, Vol. 3, No. 6, 01.06.2012, p. 1226-1240.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Broadband ultraviolet-visible optical property measurement in layered turbid media
AU - Wang, Quanzeng
AU - Le, Du
AU - Ramella-Roman, Jessica
AU - Pfefer, Joshua
PY - 2012/6/1
Y1 - 2012/6/1
N2 - The ability to accurately measure layered biological tissue optical properties (OPs) may improve understanding of spectroscopic device performance and facilitate early cancer detection. Towards these goals, we have performed theoretical and experimental evaluations of an approach for broadband measurement of absorption and reduced scattering coefficients at ultraviolet-visible wavelengths. Our technique is based on neural network (NN) inverse models trained with diffuse reflectance data from condensed Monte Carlo simulations. Experimental measurements were performed from 350 to 600 nm with a fiber-optic-based reflectance spectroscopy system. Two-layer phantoms incorporating OPs relevant to normal and dysplastic mucosal tissue and superficial layer thicknesses of 0.22 and 0.44 mm were used to assess prediction accuracy. Results showed mean OP estimation errors of 19% from the theoretical analysis and 27% from experiments. Two-step NN modeling and nonlinear spectral fitting approaches helped improve prediction accuracy. While limitations and challenges remain, the results of this study indicate that our technique can provide moderately accurate estimates of OPs in layered turbid media.
AB - The ability to accurately measure layered biological tissue optical properties (OPs) may improve understanding of spectroscopic device performance and facilitate early cancer detection. Towards these goals, we have performed theoretical and experimental evaluations of an approach for broadband measurement of absorption and reduced scattering coefficients at ultraviolet-visible wavelengths. Our technique is based on neural network (NN) inverse models trained with diffuse reflectance data from condensed Monte Carlo simulations. Experimental measurements were performed from 350 to 600 nm with a fiber-optic-based reflectance spectroscopy system. Two-layer phantoms incorporating OPs relevant to normal and dysplastic mucosal tissue and superficial layer thicknesses of 0.22 and 0.44 mm were used to assess prediction accuracy. Results showed mean OP estimation errors of 19% from the theoretical analysis and 27% from experiments. Two-step NN modeling and nonlinear spectral fitting approaches helped improve prediction accuracy. While limitations and challenges remain, the results of this study indicate that our technique can provide moderately accurate estimates of OPs in layered turbid media.
UR - http://www.scopus.com/inward/record.url?scp=84863909431&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863909431&partnerID=8YFLogxK
U2 - 10.1364/BOE.3.001226
DO - 10.1364/BOE.3.001226
M3 - Article
C2 - 22741070
AN - SCOPUS:84863909431
VL - 3
SP - 1226
EP - 1240
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 6
ER -