Broad protection with an inactivated vaccine against primary-isolated lethal enterovirus 71 infection in newborn mice

Junliang Chang, Jingliang Li, Xin Liu, Guanchen Liu, Jiaxin Yang, Wei Wei, Wenyan Zhang, Xiao Fang Yu

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Circulating enterovirus 71 (EV-A71)-associated hand, foot, and mouth disease is on the rise in the Asian-Pacific region. Although animal models have been developed using mouse-adapted EV-A71 strains, mouse models using primary EV-A71 isolates are scarce. Lethal animal models with circulating EV-A71 infection would contribute to studies of pathogenesis as well as vaccine development and evaluation. Results: In this study, we established a lethal mouse model using primary EV-A71 isolates from patients infected with serotypes that are currently circulating in humans. We also characterized the dose-dependent virulence and pathologic changes of circulating EV-A71 in this mouse model. Most importantly, we have established this mouse model as a suitable system for EV-A71 vaccine evaluation. An inactivated EV-A71 vaccine candidate offered complete protection from death induced by various circulating EV-A71 viruses to neonatal mice that were born to immunized female mice. The sera of the immunized dams and their pups showed higher neutralization titers against multiple circulating EV-A71 viruses. Conclusions: Thus, our newly established animal model using primary EV-A71 isolates is helpful for future studies on viral pathogenesis and vaccine and drug development.

Original languageEnglish (US)
Article number139
JournalBMC microbiology
Volume15
Issue number1
DOIs
StatePublished - Jul 15 2015

Keywords

  • Enterovirus 71
  • Mouse model
  • Vaccine candidate

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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