Broad profiling of DNA-binding transcription factor activities improves regulatory network construction in adult mouse tissues

Yi Min Sun, Yan Zhang, Ling Qin Zeng, Jian Ping Wu, Li Wei, Ai Hui Ren, Wei Shao, Ji Ying Qiao, Yong Chao Zhao, Liang Zhang, Keith R. Mitchelson, Jing Cheng

Research output: Contribution to journalArticlepeer-review

Abstract

Molecular systematics involves the description of the regulatory networks formed by the interconnections between active transcription factors and their target expressed genes. Here, we have determined the activities of 200 different transcription factors in six mouse tissues using an advanced mouse oligonucleotide array- based transcription factor assay (MOUSE OATFA). The transcription factor signatures from MOUSE OATFA were combined with public mRNA expression profiles to construct experimental transcriptional regulatory networks in each tissue. SRF-centered regulatory networks constructed for lung and skeletal muscle with OATFA data were confirmed by ChIP assays, and revealed examples of novel networks of expressed genes coregulated by sets of transcription factors. The combination of MOUSE OATFA with bioinformatics analysis of expressed genes provides a new paradigm for the comprehensive prediction of the transcriptional systems and their regulatory pathways in mouse.

Original languageEnglish (US)
Pages (from-to)4455-4464
Number of pages10
JournalJournal of proteome research
Volume7
Issue number10
DOIs
StatePublished - Oct 2008

Keywords

  • Microarray
  • Profiling
  • Regulatory network
  • Target gene
  • Transcription factor

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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