Brief electrical stimulation promotes the speed and accuracy of motor axonal regeneration

Abdulhakeem A. Al-Majed, Catherine M. Neumann, Thomas Brushart, Tessa Gordon

Research output: Contribution to journalArticle

Abstract

Functional recovery is often poor despite the capacity for axonal regeneration in the peripheral nervous system and advances in microsurgical technique. Regeneration of axons in mixed nerve into inappropriate pathways is a major contributing factor to this failure. In this study, we use the rat femoral nerve model of transection and surgical repair to evaluate (1) the effect of nerve transection on the speed of regeneration and the generation of motor-sensory specificity, (2) the efficacy of electrical stimulation in accelerating axonal regeneration and promoting the reinnervation of appropriate muscle pathways by femoral motor nerves, and (3) the mechanism of action of electrical stimulation. Using the retrograde neurotracers fluorogold and fluororuby to backlabel motoneurons that regenerate axons into muscle and cutaneous pathways, we found the following. (1) There is a very protracted period (10 weeks) of axonal outgrowth that adds substantially to the delay in axonal regeneration (staggered regeneration). This process of staggered regeneration is associated with preferential motor reinnervation (PMR). (2) One hour to 2 weeks of 20 Hz continuous electrical stimulation of the parent axons proximal to the repair site dramatically reduces this period (to 3 weeks) and accelerates PMR. (3) The positive effect of short-term electrical stimulation is mediated via the cell body, implicating an enhanced growth program. The effectiveness of such a short-period low-frequency electrical stimulation suggests a new therapeutic approach to accelerate nerve regeneration after injury and, in turn, improve functional recovery.

Original languageEnglish (US)
Pages (from-to)2602-2608
Number of pages7
JournalJournal of Neuroscience
Volume20
Issue number7
StatePublished - Apr 1 2000

Fingerprint

Electric Stimulation
Regeneration
Femoral Nerve
Axons
Anatomic Models
Efferent Pathways
Muscles
Nerve Regeneration
Peripheral Nervous System
Motor Neurons
Skin
Wounds and Injuries
Growth

Keywords

  • Electrical stimulation
  • Motoneuron
  • PMR
  • Retrograde labeling
  • Staggered regeneration
  • TTX

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Brief electrical stimulation promotes the speed and accuracy of motor axonal regeneration. / Al-Majed, Abdulhakeem A.; Neumann, Catherine M.; Brushart, Thomas; Gordon, Tessa.

In: Journal of Neuroscience, Vol. 20, No. 7, 01.04.2000, p. 2602-2608.

Research output: Contribution to journalArticle

Al-Majed, Abdulhakeem A. ; Neumann, Catherine M. ; Brushart, Thomas ; Gordon, Tessa. / Brief electrical stimulation promotes the speed and accuracy of motor axonal regeneration. In: Journal of Neuroscience. 2000 ; Vol. 20, No. 7. pp. 2602-2608.
@article{ecbfdaf782324f7f845df42bda53c081,
title = "Brief electrical stimulation promotes the speed and accuracy of motor axonal regeneration",
abstract = "Functional recovery is often poor despite the capacity for axonal regeneration in the peripheral nervous system and advances in microsurgical technique. Regeneration of axons in mixed nerve into inappropriate pathways is a major contributing factor to this failure. In this study, we use the rat femoral nerve model of transection and surgical repair to evaluate (1) the effect of nerve transection on the speed of regeneration and the generation of motor-sensory specificity, (2) the efficacy of electrical stimulation in accelerating axonal regeneration and promoting the reinnervation of appropriate muscle pathways by femoral motor nerves, and (3) the mechanism of action of electrical stimulation. Using the retrograde neurotracers fluorogold and fluororuby to backlabel motoneurons that regenerate axons into muscle and cutaneous pathways, we found the following. (1) There is a very protracted period (10 weeks) of axonal outgrowth that adds substantially to the delay in axonal regeneration (staggered regeneration). This process of staggered regeneration is associated with preferential motor reinnervation (PMR). (2) One hour to 2 weeks of 20 Hz continuous electrical stimulation of the parent axons proximal to the repair site dramatically reduces this period (to 3 weeks) and accelerates PMR. (3) The positive effect of short-term electrical stimulation is mediated via the cell body, implicating an enhanced growth program. The effectiveness of such a short-period low-frequency electrical stimulation suggests a new therapeutic approach to accelerate nerve regeneration after injury and, in turn, improve functional recovery.",
keywords = "Electrical stimulation, Motoneuron, PMR, Retrograde labeling, Staggered regeneration, TTX",
author = "Al-Majed, {Abdulhakeem A.} and Neumann, {Catherine M.} and Thomas Brushart and Tessa Gordon",
year = "2000",
month = "4",
day = "1",
language = "English (US)",
volume = "20",
pages = "2602--2608",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "7",

}

TY - JOUR

T1 - Brief electrical stimulation promotes the speed and accuracy of motor axonal regeneration

AU - Al-Majed, Abdulhakeem A.

AU - Neumann, Catherine M.

AU - Brushart, Thomas

AU - Gordon, Tessa

PY - 2000/4/1

Y1 - 2000/4/1

N2 - Functional recovery is often poor despite the capacity for axonal regeneration in the peripheral nervous system and advances in microsurgical technique. Regeneration of axons in mixed nerve into inappropriate pathways is a major contributing factor to this failure. In this study, we use the rat femoral nerve model of transection and surgical repair to evaluate (1) the effect of nerve transection on the speed of regeneration and the generation of motor-sensory specificity, (2) the efficacy of electrical stimulation in accelerating axonal regeneration and promoting the reinnervation of appropriate muscle pathways by femoral motor nerves, and (3) the mechanism of action of electrical stimulation. Using the retrograde neurotracers fluorogold and fluororuby to backlabel motoneurons that regenerate axons into muscle and cutaneous pathways, we found the following. (1) There is a very protracted period (10 weeks) of axonal outgrowth that adds substantially to the delay in axonal regeneration (staggered regeneration). This process of staggered regeneration is associated with preferential motor reinnervation (PMR). (2) One hour to 2 weeks of 20 Hz continuous electrical stimulation of the parent axons proximal to the repair site dramatically reduces this period (to 3 weeks) and accelerates PMR. (3) The positive effect of short-term electrical stimulation is mediated via the cell body, implicating an enhanced growth program. The effectiveness of such a short-period low-frequency electrical stimulation suggests a new therapeutic approach to accelerate nerve regeneration after injury and, in turn, improve functional recovery.

AB - Functional recovery is often poor despite the capacity for axonal regeneration in the peripheral nervous system and advances in microsurgical technique. Regeneration of axons in mixed nerve into inappropriate pathways is a major contributing factor to this failure. In this study, we use the rat femoral nerve model of transection and surgical repair to evaluate (1) the effect of nerve transection on the speed of regeneration and the generation of motor-sensory specificity, (2) the efficacy of electrical stimulation in accelerating axonal regeneration and promoting the reinnervation of appropriate muscle pathways by femoral motor nerves, and (3) the mechanism of action of electrical stimulation. Using the retrograde neurotracers fluorogold and fluororuby to backlabel motoneurons that regenerate axons into muscle and cutaneous pathways, we found the following. (1) There is a very protracted period (10 weeks) of axonal outgrowth that adds substantially to the delay in axonal regeneration (staggered regeneration). This process of staggered regeneration is associated with preferential motor reinnervation (PMR). (2) One hour to 2 weeks of 20 Hz continuous electrical stimulation of the parent axons proximal to the repair site dramatically reduces this period (to 3 weeks) and accelerates PMR. (3) The positive effect of short-term electrical stimulation is mediated via the cell body, implicating an enhanced growth program. The effectiveness of such a short-period low-frequency electrical stimulation suggests a new therapeutic approach to accelerate nerve regeneration after injury and, in turn, improve functional recovery.

KW - Electrical stimulation

KW - Motoneuron

KW - PMR

KW - Retrograde labeling

KW - Staggered regeneration

KW - TTX

UR - http://www.scopus.com/inward/record.url?scp=0034175684&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034175684&partnerID=8YFLogxK

M3 - Article

VL - 20

SP - 2602

EP - 2608

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 7

ER -