TY - JOUR
T1 - Bridging Conformational Dynamics and Function Using Single-Molecule Spectroscopy
AU - Myong, Sua
AU - Stevens, Benjamin C.
AU - Ha, Taekjip
N1 - Funding Information:
We thank Sean McKinney, Michelle Nahas, Michael Börsch, and Scott Blanchard for helpful suggestions on the manuscript. Funding during the preparation of this manuscript was provided by the National Institutes of Health.
PY - 2006/4
Y1 - 2006/4
N2 - In a typical structure-function relation study, the primary structure of proteins or nucleic acids is changed by mutagenesis and its functional effect is measured via biochemical means. Single-molecule spectroscopy has begun to give a whole new meaning to the "structure-function relation" by measuring the real-time conformational changes of individual biological macromolecules while they are functioning. This review discusses a few recent examples: untangling internal chemistry and conformational dynamics of a ribozyme, branch migration landscape of a Holliday junction at a single-step resolution, tRNA selection and dynamics in a ribosome, repetitive shuttling and snapback of a helicase, and discrete rotation of an ATP synthase.
AB - In a typical structure-function relation study, the primary structure of proteins or nucleic acids is changed by mutagenesis and its functional effect is measured via biochemical means. Single-molecule spectroscopy has begun to give a whole new meaning to the "structure-function relation" by measuring the real-time conformational changes of individual biological macromolecules while they are functioning. This review discusses a few recent examples: untangling internal chemistry and conformational dynamics of a ribozyme, branch migration landscape of a Holliday junction at a single-step resolution, tRNA selection and dynamics in a ribosome, repetitive shuttling and snapback of a helicase, and discrete rotation of an ATP synthase.
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U2 - 10.1016/j.str.2006.02.005
DO - 10.1016/j.str.2006.02.005
M3 - Review article
C2 - 16615904
AN - SCOPUS:33645990641
SN - 0969-2126
VL - 14
SP - 633
EP - 643
JO - Structure
JF - Structure
IS - 4
ER -