Breath ammonia and ethanol increase in response to a high protein challenge

Lisa A. Spacek; Matthew L. Mudalel; Rafal Lewicki; Frank K. Tittel; Terence H. Risby; Jill Stoltzfus; Joseph J. Munier; Steven F. Solga

doi:10.3109/1354750X.2015.1040840

Breath ammonia and ethanol increase in response to a high protein challenge

Lisa A. Spacek, Matthew L. Mudalel, Rafal Lewicki, Frank K. Tittel, Terence H. Risby, Jill Stoltzfus, Joseph J. Munier, Steven F. Solga

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10 Scopus citations

Abstract

Quantifying changes in ammonia and ethanol in blood and body fluid assays in response to food is cumbersome. We used breath analysis of ammonia, ethanol, hydrogen (an accepted standard of gut transit) and acetone to investigate gastrointestinal physiology. In 30 healthy participants, we measured each metabolite serially over 6h in control and high protein trials. Two-way repeated measures ANOVA compared treatment (control versus intervention), change from baseline to maximum and interaction of treatment and time change. Interaction was significant for ammonia (p<0.0001) and hydrogen (p<0.0001). We describe the dynamic measurement of multiple metabolites in response to an oral challenge.

Original language	English (US)
Pages (from-to)	149-156
Number of pages	8
Journal	Biomarkers
Volume	20
Issue number	2
DOIs	https://doi.org/10.3109/1354750X.2015.1040840
State	Published - Mar 1 2015

Keywords

Acetone
Ammonia
Breath metabolites
Ethanol
Gastrointestinal physiology

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Health, Toxicology and Mutagenesis

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10.3109/1354750X.2015.1040840

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abstract = "Quantifying changes in ammonia and ethanol in blood and body fluid assays in response to food is cumbersome. We used breath analysis of ammonia, ethanol, hydrogen (an accepted standard of gut transit) and acetone to investigate gastrointestinal physiology. In 30 healthy participants, we measured each metabolite serially over 6h in control and high protein trials. Two-way repeated measures ANOVA compared treatment (control versus intervention), change from baseline to maximum and interaction of treatment and time change. Interaction was significant for ammonia (p<0.0001) and hydrogen (p<0.0001). We describe the dynamic measurement of multiple metabolites in response to an oral challenge.",

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AU - Spacek, Lisa A.

AU - Mudalel, Matthew L.

AU - Lewicki, Rafal

AU - Tittel, Frank K.

AU - Risby, Terence H.

AU - Stoltzfus, Jill

AU - Munier, Joseph J.

AU - Solga, Steven F.

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N2 - Quantifying changes in ammonia and ethanol in blood and body fluid assays in response to food is cumbersome. We used breath analysis of ammonia, ethanol, hydrogen (an accepted standard of gut transit) and acetone to investigate gastrointestinal physiology. In 30 healthy participants, we measured each metabolite serially over 6h in control and high protein trials. Two-way repeated measures ANOVA compared treatment (control versus intervention), change from baseline to maximum and interaction of treatment and time change. Interaction was significant for ammonia (p<0.0001) and hydrogen (p<0.0001). We describe the dynamic measurement of multiple metabolites in response to an oral challenge.

AB - Quantifying changes in ammonia and ethanol in blood and body fluid assays in response to food is cumbersome. We used breath analysis of ammonia, ethanol, hydrogen (an accepted standard of gut transit) and acetone to investigate gastrointestinal physiology. In 30 healthy participants, we measured each metabolite serially over 6h in control and high protein trials. Two-way repeated measures ANOVA compared treatment (control versus intervention), change from baseline to maximum and interaction of treatment and time change. Interaction was significant for ammonia (p<0.0001) and hydrogen (p<0.0001). We describe the dynamic measurement of multiple metabolites in response to an oral challenge.

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KW - Ethanol

KW - Gastrointestinal physiology

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Breath ammonia and ethanol increase in response to a high protein challenge

Abstract

Keywords

ASJC Scopus subject areas

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