Brain uptake of α‐[14C]methyl‐para‐tyrosine in the rat

P. Cumming, T. K. Venkatachalam, S. Rajagopal, M. Diksic, A. Gjedde

Research output: Contribution to journalArticle

Abstract

The blood‐brain permeabilities of L‐[3H]tyrosine and the tyrosine hydroxylase (TH) inhibitor β‐[14C]methyl‐para‐tyrosine ([14C]AMPT) were determined in rat striatum, a brain region rich in TH activity, and in other brain regions containing relatively little TH activity. In striatum, the unidirectional clearance rate (K1) for L‐[3H]tyrosine (6.2 ml hg‐ −1 min−1) was significantly greater than the rates for L‐[14C]AMPT (2.8 ml hg−1 min−1) and D‐[14C]AMPT (0.8 ml hg−1 min−1). The apparent volume of distribution (Vf) for L‐[14C]AMPT in striatum (72.5 ± 4.0 ml hg‐1) did not differ from the Vf in other brain regions. The homogeneous distribution of L‐[14C]AMPT in rat brain indicates that labeled AMPT is unsuitable for the study of TH in vivo by quantitative autoradiography. © 1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)125-128
Number of pages4
JournalSynapse
Volume17
Issue number2
DOIs
StatePublished - Jun 1994

Keywords

  • Brain
  • Inhibitors
  • Positron emission tomography
  • Radiotracers
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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    Cumming, P., Venkatachalam, T. K., Rajagopal, S., Diksic, M., & Gjedde, A. (1994). Brain uptake of α‐[14C]methyl‐para‐tyrosine in the rat. Synapse, 17(2), 125-128. https://doi.org/10.1002/syn.890170208